• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Recombinant Mouse Podoplanin Fc Chimera (Catalog # ) is coated at 0.5 μg/mL (100 μL/well), Recombinant Mouse    
  CLEC-2/CLEC1B binds with a typical ED    ₅₀ of    
  0.1-0.5 μg/mL.

• Source

  Mouse myeloma cell line, NS0-derived Met53-Leu229, with an N-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  His

• Predicted Molecular Mass

  21 kDa

• SDS-PAGE

  24-39 kDa, reducing conditions

Data Images

Background: CLEC-2/CLEC1B

C-type lectin-like receptor 2, also known as CLEC-1B, is a 32 kDa type II transmembrane glycoprotein that is expressed on platelets, megakaryocyte, and liver sinusoidal cells (1-4). Mature mouse CLEC-2 consists of a 31 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) with one C-type lectin domain. Within the ECD, mouse CLEC-2 shares 59% and 90% aa sequence identity with human and rat CLEC-2, respectively. Alternative splicing generates a short isoform that lacks the transmembrane segment. CLEC-2 binding to Podoplanin trigger platelet activation, shedding of GPVI, and cleavage of Fcg RIIA/CD32a (5-8). CLEC-2 expression on platelets is required for the development of lymphatic vessels and valves as well as the maintenance of lymph node high endothelial veinules (HEV) during lymphocyte crossing (7-10). CLEC-2 additionally binds to fucoidan, HIV-1, and the platelet-aggregating snake venom protein Rhodocytin/Aggretin (2, 3, 11). In the liver, soluble CLEC-2 promotes M2 macrophage differentiation and limits inflammatory cytokine production (12).

• References:

1. Lee, R.H. and W. Bergmeier (2016) J. Thromb. Haemost. Epub PMID 26749528.

2. Suzuki-Inoue, K. et al. (2006) Blood 107:542.

3. Chaipan, C. et al. (2006) J. Virol. 80:8951.

4. Gitz, E. et al. (2014) Blood 124:2262.

5. Suzuki-Inoue, K. et al. (2007) J. Biol. Chem. 282:25993.

6. Christou, C.M. et al. (2008) Biochem. J. 411:133.

7. Herzog, B.H. et al. (2013) Nature 502:105.

8. Sweet, D.T. et al. (2015) J. Clin. Invest. 125:2995.

9. Hess, P.R. et al. (2014) J. Clin. Invest. 124:273.

10. Osada, M. et al. (2012) J. Biol. Chem. 287:22241.

11. Manne, B.K. et al. (2013) J. Biol. Chem. 288:7717.

12. Wu, X. et al. (2015) EbioMedicine 2:214.

• Long Name:

  C-type Lectin-like Receptor 2

• Entrez Gene IDs:

  51266 (Human); 56760 (Mouse)

• Alternate Names:

  1810061I13Rik; CLEC1B; CLEC2; CLEC-2; CLEC2B; CLEC2PRO1384; C-type lectin domain family 1 member B; C-type lectin domain family 1, member B; C-type lectin-like receptor 2; C-type lectin-like receptor-2; QDED721