• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Recombinant Mouse TGF- beta 2 (Catalog # ) is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse Vasorin/SLIT-like 2 that produces 50% of the optimal binding response is approximately 0.3-1.8 μg/mL

• Source

  Mouse myeloma cell line, NS0-derived Cys25-Pro576, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Cys25

• Predicted Molecular Mass

  61 kDa

• SDS-PAGE

  72-97 kDa, reducing conditions    
   

Background: Vasorin/SLIT-like 2

Vasorin, also known as SLIT-like 2, is an approximately 110 kDa transmembrane protein that is expressed on vascular smooth muscle cells and in the developing skeletal system (1, 2). It is down-regulated following vascular injury (1). A 95 kDa soluble form of Vasorin can be shed from the cell surface by ADAM17 mediated cleavage (3). The shed soluble form of Vasorin binds to TGF-beta 1, 2, and 3 and prevents TGF-beta induced effects including intimal formation and epithelial mesenchymal transition (1, 3). Soluble Vasorin is elevated in the serum of hepatic carcinoma patients relative to normal or non-cancerous hepatic disease (4). It promotes tumor cell proliferation, migration, and survival (4). Mature mouse Vasorin consists of a 552 amino acid (aa) extracellular domain (ECD) with 10 leucine rich repeats (LRR) flanked by one LRRNT and one LRRCT domain, one EGF-like domain, and one Fibronectin type-III domain, a 21 aa transmembrane segment, and a 76 aa cytoplasmic domain (1). Within the ECD, mouse Vasorin shares 85% and 96% aa sequence identity with human and rat Vasorin, respectively.

• References:

1. Ikeda, Y. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10732.

2. Krautzberger, A.M. et al. (2012) Gene Expr. Patterns 12:167.

3. Malapeira, J. et al. (2011) Oncogene 30:1912.

4. Li, S. et al. (2015) Oncotarget 6:10045.

• Entrez Gene IDs:

  114990 (Human); 246154 (Mouse)

• Alternate Names:

  Atia; Protein slit-like 2; SLIT like 2; Slitl2; slit-like 2 (Drosophila); slit-like 2; VASN; Vasorin