• Purity

  >95%, by SDS-PAGE with silver staining

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of the MCF?7 human breast cancer cells. The ED    ₅₀ for this effect is 0.25-1 μg/mL.

• Source

  Mouse myeloma cell line, NS0-derived Asp157-Val709, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Asp157

• Predicted Molecular Mass

  62 kDa

• SDS-PAGE

  75-87 kDa, reducing conditions

Background: E-Cadherin

E-Cadherin/Cadherin-1, also known as Uvomorulin in the mouse and rat, is a 120 kDa member of the Cadherin family of cell surface glycoproteins that mediate cell adhesion (1). Mouse E-Cadherin shares 92% and 81% amino acid sequence identity with the rat and human proteins, respectively (2). It is a single-pass transmembrane protein that mediates calcium-dependent epithelial cell adhesion. E-Cadherin has five extracellular EC domains that form homophilic cis-clusters between adjacent epithelial cells and trans-clusters within the same cell. E-Cadherin clusters are critical components of adherens junctions between epithelial cells and act in the formation and maintenance of the epithelial cell barrier (3, 4). The intracellular domain of E-Cadherin binds to the Catenin cytoskeletal complex, which includes p120 Catenin, beta-Catenin, alpha-Catenin, and Vinculin. E-Cadherin expression is critical for epithelial tissue homeostasis. Decreased E-Cadherin is associated with physiological and pathological epithelial-to-mesenchymal transition and cell migration, and E-Cadherin loss contributes to cancer metastasis (5). The extracellular E-Cadherin N-terminal domain can be cleaved by several proteases and is released as a soluble factor that enhances cancer cell motility and EGFR-dependent survival and proliferation (6).

• References:

1. Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.

2. Nagafuchi, A. et al. (1987) Nature 329:341.

3. Guillot, C. and T. Lecuit (2013) Science 340:1185.

4. Tian, X. et al. (2011) J. Biomed. Biotechnol. 2011:567305.

5. Stemmler, M.P. (2008) Mol. Biosyst. 4:835.

6. David, J.M. and A.K. Rajasekaran (2012) Cancer Res. 72:2917.

• Entrez Gene IDs:

  999 (Human); 12550 (Mouse); 83502 (Rat)

• Alternate Names:

  Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin