• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to bind biotinylated rhBLAME in a functional ELISA.

• Source

  Mouse myeloma cell line, NS0-derived Ala23-Asp233, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Ala23

• Predicted Molecular Mass

  24.7 kDa

• SDS-PAGE

  30 - 40 kDa, reducing conditions

Background: BLAME/SLAMF8

BLAME (B-lymphocyte activator macrophage expressed), also known as SLAM family member 8, is a type I transmembrane protein that belongs to the CD2 subset of immunoglobulin superfamily cell receptors. CD2 family proteins function as adhesion molecules and modulators of immune responses (1, 2). Mature human BLAME consists of a 211 amino acid (aa) ECD that contains two Ig V-like domains, a 21 aa transmembrane segment, and a 31 aa cytoplasmic tail that lacks recognizable signaling motifs (3). Within the ECD, human BLAME shares 19% - 26% aa sequence identity with human 2B4, CD2, CD2F-10, CD48, CD58, CD84, CD229, CRACC, NTB-A, and SLAM. It shares 79% aa sequence identity with the ECD of mouse BLAME. BLAME is expressed on dendritic cells and IFN-gamma stimulated monocytes. Overexpression of BLAME in bone marrow cells leads to an increase in the peritoneal B1b population of B lymphocytes (3).

• References:

1. McNerney, M.E. and V. Kumar (2006) Curr. Top. Microbiol. Immunol. 298:91.

2. Veillette, A. (2006) Nat. Rev. Immunol. 6:56.

3. Kingsbury, G.A. et al. (2001) J. Immunol. 166:5675.

• Entrez Gene IDs:

  56833 (Human)

• Alternate Names:

  B Lymphocyte Activator Macrophage Expressed; BCM-Like Membrane Protein; BLAME; B-Lymphocyte Activator Macrophage Expressed; CD353 Antigen; CD353; SBBI42; SLAM Family Member 8; SLAMF8