• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. Welte, S.     et al. (2006) Nat. Immunol.     7:12. rhNKp80/Fc Chimera, immobilized at 5 μg/mL (100 μL/well), can induce greater than 40% of U937 cell adhesion.

• Source

  Mouse myeloma cell line, NS0-derived

  MD	Human IgG1 (Pro100 - Lys330)	IEGR	Human NKp80 (Val66 - Tyr231) Accession # Q9NZS2
  N-terminus			C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Met

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  45.8 kDa (monomer)

• SDS-PAGE

  59-62 kDa, reducing conditions

Background: NKp80/KLRF1

NKp80, also known as killer cell lectin-like receptor subfamily F member 1 (KLRF1), is an 80 kDa type II transmembrane, homodimeric, activating coreceptor expressed exclusively on the surface of NK cells. Human NKp80, a member of the C-type lectin family of proteins (1 - 3), is 231 aa in length with a 38 amino acid (aa) cytoplasmic region, a 21 aa transmembrane region, and a 172 aa extracellular domain (ECD) (SwissProt # Q9NZS2). The ECD contains the C-type lectin-like domain (aa 114 - 230) and four potential sites of N-linked glycosylation. Multiple splicing variants produce four isoforms for NKp80. Isoform 1 is the standard protein. There are no murine orthologs for NKp80 (4). One early study suggested that NKp80 functions as an inhibitory receptor based on the presence of two immunoreceptor tyrosine-based inhibitory motifs (ITIM) in the N-terminal cytoplasmic region, and the lack of charged amino acids in the transmembrane region (1). However, later studies show that NKp80 displays an activating function rather than an inhibitory one (2 - 3, 5). In in vitro experiments in human NK cells, NKp80 stimulates NK cell cytotoxicity and induces calcium influx after triggering by appropriate antibodies (2 - 3). One study reports that NKp80 likely functions as a coreceptor, cooperating with NKp46 and other activating receptors in NK cell cytotoxicity (2). Activation-induced C-type lectin (AICL) is the ligand for NKp80 (3). It is a myeloid-specific receptor expressed by monocytes, macrophages, and granulocytes (3). Crosslinking of both NKp80 and AICL stimulates secretion of proinflammatory cytokines suggesting that NKp80-AICL interaction is involved in the activating crosstalk between NK cells and myeloid cells, and thus may influence the initiation and maintenance of immune responses in humans (3).

• References:

1. Roda-Navarro, P. et al. (2000) Eur. J. Immunol. 30:568.

2. Vitale, M. et al. (2001) Eur. J. Immunol. 31:233.

3. Welte, S. et al. (2006) Nat. Immunol. 7:1334.

4. Yokoyama, W.M. and B.F. Plougastel (2003) Nat. Rev. Immunol. 3:304.

5. Thomas, M. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105:1656.

• Entrez Gene IDs:

  51348 (Human)

• Alternate Names:

  Activating coreceptor NKp80; CLEC5C; CLEC5CMGC119908; C-type lectin domain family 5 member C; killer cell lectin-like receptor F1; killer cell lectin-like receptor subfamily F member 1; killer cell lectin-like receptor subfamily F, member 1; KLRF1; Lectin-like receptor F1; MGC119907; MGC119909; ML; NKp80