• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to bind biotinylated Laminarin (1, 3-beta -glucan) in a functional ELISA with an estimated K    _D <5 nM.  

• Source

  Mouse myeloma cell line, NS0-derived Thr66-Met201, with an N-terminal 10-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  His

• Predicted Molecular Mass

  16.8 kDa

Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3 - 5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4⁺ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5 - 7). Yeast beta -glucan is accessible to Dectin-1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8 - 10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11 - 13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15 - 16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29% - 39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.

• References:

1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.

2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.

3. Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288.

4. Yokota, K. et al. (2001) Gene 272:51.

5. Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818.

6. Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539.

7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.

8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.

9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.

10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.

11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.

12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.

13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.

14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.

15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.

16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.

• Entrez Gene IDs:

  64581 (Human); 56644 (Mouse)

• Alternate Names:

  Beta-glucan receptor; BGR; CD369; CLEC7A; CLECSF12; CLECSF12DC-associated C-type lectin 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; Dectin1; Dectin-1; DECTIN1CANDF4; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; hDectin-1; lectin-like receptor 1