• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <1.0 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit BAFF-mediated proliferation of anti-IgM stimulated mouse B cells. Moore, P.A.     et al. (1999) Science     285:260; Gross, J.A.     et al. (2000) Nature     404:995; Schneider, P.     et al. (1999) J. Exp. Med.     189:1747. The ED    ₅₀ for this effect is 3?10 ng/mL.

• Source

  Mouse myeloma cell line, NS0-derived

  Human TACI (Ser2-Thr166) Accession # Q53F36	DIEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Ser2, Ser123

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  45.2 kDa (starts at Ser2, monomer)31.3 kDa (starts at Ser123, monomer)

• SDS-PAGE

  51 kDa and 40 kDa, reducing conditions

Background: TACI/TNFRSF13B

TACI, transmembrane activator and CAML-interactor, is a member of the TNF receptor superfamily. TACI is a type III membrane protein with an extracellular N?terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine rich domains. Within the TNFRSF, it shares the highest homology with BCMA. TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Human TACI is a 293 amino acid (aa) protein consisting of a 166 aa extracellular domain, a 20 aa transmembrane domain, and a 107 aa intracellular domain. Human and mouse TACI share 54% amino acid identity.

• References:

1. Xia, X.-Z. et al. (2000) J. Exp. Med. 192:137.

2. von Bulow, G.U. et al. (1997) Science, 278:138.

3. Gross, J.A. et al. (2000) Nature 404:995.

4. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.

5. Yan, M. et al. (2000) Nature Immunol. 1:37.

6. Wu, Y. et al. (2000) J. Biol. Chem. 275:35478.

• Long Name:

  Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B

• Entrez Gene IDs:

  23495 (Human); 57916 (Mouse)

• Alternate Names:

  CD267 antigen; CD267; CVID; CVID2; FLJ39942; MGC133214; TACI; TACIMGC39952; TNFRSF13B; TNFRSF14B; Transmembrane activator and CAML interactor; tumor necrosis factor receptor 13B; tumor necrosis factor receptor superfamily member 13B; tumor necrosis factor receptor superfamily, member 13B