• Purity

  >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M.     et al. (2005) J. Biol. Chem.     280:15842. The ED    ₅₀ for this effect is 0.5-2 μg/mL.

• Source

  Chinese Hamster Ovary cell line, CHO-derived

  Human Jagged 2 (Met27-Leu1082) Accession #AAB61285	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Met27

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  138.7 kDa (monomer)

• SDS-PAGE

  165-175 kDa, reducing conditions

Background: Jagged 2

Human Jagged 2 is a 131 kDa (predicted) member of the Delta-Serrate-Lag-2 (DSL) family of ligands. This family activates LIN12/Notch proteins and thereby regulates cell fate determination during development (1 - 5). It is a type 1 transmembrane protein that is synthesized as a 1238 amino acid (aa) precursor (SwissProt # Q9Y219). It contains a 23 aa signal sequence, a large 1057 aa extracellular region, a 21 aa transmembrane region, and a short 137 aa cytoplasmic region. The extracellular region contains four potential N-linked glycosylation sites, a DSL domain, 16 EGF-like repeats (many of which are also sites of calcium binding), a von Willebrand factor (vWF) type C domain, and a cysteine-rich region just proximal to the transmembrane segment (2). There are two isoforms for human Jagged 2, named long and short. The short form lacks a splicing variant region (aa 421 - 461) that is present in the long form of the protein. Human Jagged 2 shares 90% and 87% aa sequence identity with mouse and rat Jagged 2, respectively. During murine embryonic development, Jagged 2 is expressed highest in fetal thymus, epidermis, foregut, dorsal root ganglia, and inner ear (2). In 2-week old mice, the Jagged 2 transcript is most abundant in heart, lung, thymus, skeletal muscle, brain, and testis (2). Functionally, it is suggested that Jagged 2 engages the Notch1 pathway of signal transduction (2). It is involved in the development of the mammalian limb, branchial arches, central and peripheral nervous systems, T cell lineage differentiation, natural killer cells, and the establishment of functional natural killer cell lines (3, 5, 6).

• References:

1. Shawber, C. et al. (1996) Dev. Biol. 180:370.

2. Luo, B. et al. (1997) Mol. Cell. Biol. 17:6057.

3. Valsecchi, V. et al. (1997) Mech. Dev. 69:203.

4. Schickwann, T. et al. (2000) Blood 96:950.

5. DeHart, S. et al. (2005) Blood 105:3521.

6. de La Coste, A. and A.A. Freitas (2006) Immunol. Lett. 102:1.

• Entrez Gene IDs:

  3714 (Human); 16450 (Mouse); 29147 (Rat)

• Alternate Names:

  HJ2; JAG2; Jagged 2; Jagged2; protein jagged-2; SER2