• Species Reactivity

  Human

• Specificity

  Detects human TROP-2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) VCAM?1 or rhICAM?1 is observed.

• Source

  Monoclonal Mouse IgG    _2A Clone # 77220

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human TROP-2    
  His27-Thr274    
  Accession # P09758

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TROP-2

Human TROP-2, also known as Tumor Associated Calcium Signal Transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

• References:

1. Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27.

2. Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507.

3. Fornaro, M. et al. (1995) Int. J. Cancer 62:610.

4. Ripani, E. et al. (1998) Int. J. Cancer 76:671.

5. El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.

• Long Name:

  Tumor-associated Calcium Signal Transducer 2

• Entrez Gene IDs:

  4070 (Human); 56753 (Mouse)

• Alternate Names:

  Cell surface glycoprotein Trop-2; EGP1; EGP-1; epithelial glycoprotein-1; GA733-1; GA733-1GP50; gastrointestinal tumor-associated antigen GA7331; gp50; M1S1cell surface glycoprotein TROP2; Membrane component chromosome 1 surface marker 1; membrane component, chromosome 1, surface marker 1; Pancreatic carcinoma marker protein GA733-1; pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733; T16; TACSTD2; TROP2; TROP-2; TROP2GA7331; tumor-associated calcium signal transducer 2