• Species Reactivity

  Human

• Specificity

  Detects human Lgr5/GPR49 in ELISAs. Stains human and mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry and Immunocytochemistry. This antibody, also known as "RD42", has been found to detect an epitope in the C-terminal LRR cap of Lgr5 (Ref. 1).    
   

• Source

  Monoclonal Mouse IgG    _2A Clone # 707042

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human Lgr5/GPR49    
  Met1-Ile560    
  Accession # O75473

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Lgr5/GPR49

Leucine?rich repeat G?protein?coupled receptor 5 (Lgr5), also called GPR49, is a 907 amino acid (aa), approximately 97 kDa (calculated), seven?transmembrane glycoprotein receptor in the Lgr family of cell surface receptors. The subfamily of Lgrs comprising Lgr4, Lgr5, and Lgr6 are G?protein?independent mediators of the potentiating effect of R?Spondins on Wnt signaling (2). Lgr5 binds and forms complexes with R?Spondins, Frizzled Wnt receptors and LRP Wnt co?receptors. The region of the human Lgr5 long extracellular domain used as an immunogen shares 90% amino acid sequence identity with mouse and rat Lgr5, respectively. Lgr5 is found on embryonic and adult epithelial stem cells (3). Lgr5  ⁺ stem cells can produce all epithelial cell types of the intestinal crypts (4). Abnormal Lgr5 expression and regulation in stem cells might give rise to cancers such as intestinal, hepatocellular, pancreatic and ovarian carcinomas (5,6). This antibody has been referred to as "RD42" in Peng   et al. (1).  

• References:

1. Peng et al. (2013) Cell Rep. 3(6):1885.

2. de Lau et al. (2014) Genes Dev. 28(4):305.

3. Barker et al. (2013) Development 140(12):2484.

4. Clevers (2013) Nature 495(7439):53.

5. Wu et al. (2014) Nat Commun 5:3149.

6. Jang et al. (2013) PLoS One 8(12): e82390495.

• Long Name:

  Leucine-rich Repeat Containing G Protein-coupled Receptor 5

• Entrez Gene IDs:

  8549 (Human); 14160 (Mouse); 299802 (Rat)

• Alternate Names:

  FEX; G protein-coupled receptor 49; GPR49; GPR49G-protein coupled receptor HG38; GPR67; G-protein coupled receptor 49; G-protein coupled receptor 67; GRP49; HG38; leucine-rich repeat containing G protein-coupled receptor 5; leucine-rich repeat-containing G protein-coupled receptor 5; leucine-rich repeat-containing G-protein coupled receptor 5; Lgr5; MGC117008; orphan G protein-coupled receptor HG38