• Species Reactivity

  Human

• Specificity

  Detects human CD155/PVR in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG1 Clone # 300907

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human CD155/PVR    
  Gly27-Asn343    
  Accession # AAH15542

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 μg/10    ⁶ cells

  U937 human histiocytic lymphoma cell line and HUVEC human umbilical vein endothelial cells

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD155/PVR

CD155 [also known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5)] is a 70 kDa type I transmembrane (TM) glycoprotein that is a member of the nectin-like (Necl) family of nectin-related molecules (1). Like nectins, Necl molecules are Ig superfamily members that contain three Ig-like extracellular domains, a TM segment, and a cytoplasmic tail. Unlike nectins, Necl molecules cannot interact with cytoplasmic afadin (1). While Nectins serve as cell adhesion molecules, the actual functions of most Necls are yet-to-be determined. CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells (2, 3). The full-length (or CD155 alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20 aa signal sequence, a 323 aa extracellular region, a 24 aa TM segment and a 50 aa cytoplasmic tail. The extracellular region contains one N-terminal V-type and two C2-type Ig-like domains (2, 3). The V-type domain mediates polio virus binding (4). Three other isoforms exist, all of which retain the Ig-like domains. CD155δ is transmembrane with a shortened cytoplasmic tail of 25 aa. CD155 beta (352 aa) and CD155 gamma (344 aa) are 60?65 kDa soluble forms that show removal of the TM segment and surrounding amino acids (2, 5). The soluble forms will bind the polio virus (due to the presence of the V-type Ig domain) but afford no protection against polio infection because of low circulating levels (5). CD155 has been demonstrated to bind vitronectin, nectin-3, and DNAM-1 (6?8). DNAM-1 binding promotes monocyte migration and NK cell killing. CD155 is expressed in all normal tissues and is highly expressed in tumor cells of epithelial and neuronal origin.

• References:

1. Takai, Y. et al. (2003) Cancer Sci. 94:655.

2. Mendelsohn, C.L. et al. (1989) Cell 56:855.

3. Koike, H. et al. (1990) EMBO J. 9:3217.

4. Koike, S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4104.

5. Baury, B. et al. (2003) Biochem. Biophys. Res. Commun. 309:175.

6. Mueller, S. and E. Wimmer (2003) J. Biol. Chem. 278:31251.

7. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.

8. Lange, R. et al. (2001) Virology 285:218.

• Long Name:

  Poliovirus Receptor

• Entrez Gene IDs:

  5817 (Human); 52118 (Mouse); 25066 (Rat)

• Alternate Names:

  CD155 antigen; CD155; HVED; NECL5; Necl-5; nectin-like 5; Nectin-like protein 5; poliovirus receptor; PVR; PVS; PVSFLJ25946; Tage4