• Species Reactivity

  Mouse

• Specificity

  Detects mouse CD63 in direct ELISAs.

• Source

  Monoclonal Rat IgG1 Clone # 446703

• Immunogen

  HEK293 human embryonic kidney cell line transfected with mouse CD63    
  Met1-Met238    
  Accession # P41731

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 405

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 μg/10    ⁶ cells

  HEK293 human embryonic kidney cell line transfected with mouse CD63 and eGFP

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD63

CD63, also known as LAMP-3 or ME491 (melanoma-associated antigen), is a 30-60 kDa member of the tetraspanin superfamily of protein trafficking proteins. CD63 is ubiquitously expressed and found in late endocytic vesicles, but following cell activation is also present on the plasma membrane. Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration and degranulation. Extracellular regions of mouse CD63 share 94% and 67% amino acid sequence identity with rat and human CD63, respectively.

• Entrez Gene IDs:

  967 (Human); 12512 (Mouse); 29186 (Rat); 474391 (Canine)

• Alternate Names:

  CD63 antigen (melanoma 1 antigen); CD63 antigen; CD63 molecule; CD63; Granulophysin; Lamp-3; Lysosomal-associated membrane protein 3; ME491; melanoma 1 antigen; Melanoma-associated antigen ME491; melanoma-associated antigen MLA1; MLA1lysosome-associated membrane glycoprotein 3; Ocular melanoma-associated antigen; OMA81H; tetraspanin-30; Tspan30; tspan-30; TSPAN30granulophysin