• Species Reactivity

  Mouse

• Specificity

  Detects mouse CD28 in ELISA.

• Source

  Monoclonal Rat IgG1 Clone # 794716

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse CD28    
  Accession # P31041

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 405

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 μg/10    ⁶ cells

  CD3    ⁺ mouse splenocytes

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD28

CD28 and CTLA-4, together with their ligands B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD28 and CTLA-4 are composed of a single Ig
V?like extracellular domain, a transmembrane domain and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2 and mouse chromosome 1. Mouse CD28 is expressed constitutively on virtually 100% of mouse T cells and on developing thymocytes. Cell surface expression of mouse CD28 is down-regulated upon ligation of CD28 in the presence of PMA or PHA. In contrast, CTLA-4 is not expressed constitutively but is upregulated rapidly following T cell activation and CD28 ligation. Cell surface expression of CTLA-4 peaks approximately 48 hours after activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to
B7?1 and B7?2 with a 20-100 fold higher affinity than CD28. CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the up-regulation of
Bcl?x_L. CD28 ligation has also been shown to regulate Th1/Th2 differentiation. Agonist activity has been reported using MAB4831 (4,5).

• References:

1. Lenschow, D.J. et al. (1996) Annu. Rev. Immunol. 14:233.

2. Hathcock, K.S. and R.J. Hodes (1996) Advances in Immunol. 62:131.

3. Ward, S.G. (1996) Biochem. J. 318:361.

4. Nguyen, P. et al. (2003) Blood 13:4320.

5. Orbach, A. et al. (2007) J. Immunol. 179:7287.

• Entrez Gene IDs:

  940 (Human); 12487 (Mouse); 102146670 (Cynomolgus Monkey)

• Alternate Names:

  CD28 antigen (Tp44); CD28 antigen; CD28 molecule; CD28; MGC138290; T-cell-specific surface glycoprotein CD28; Tp44