• Species Reactivity

  Human

• Specificity

  Detects human SH2B1 in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG    _2A Clone # 700320

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human SH2B1    
  Pro317-Leu467    
  Accession # Q9NRF2

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SH2B1

SH2B1 (SH2 adaptor protein B1), also called PSM (Pro-rich, PH and SH2 domain-containing signaling mediator) is an adaptor protein that mediates signaling between receptor tyrosine kinases and JAK signaling pathways. The 756 amino acid (aa) human SH2B1 contains an N-terminal Phe-zipper dimerization domain (aa 26-82), a nuclear localization sequence (aa 224-233), a PH domain (aa 267-376), and a pY-binding SH2 domain (aa 526-614). Within the region used as an immunogen, human SH2B1 shares 92% aa sequence identity with human and mouse SH2B1. Potential isoforms of 683 and 671 diverge after aa 632; a potential 426 aa form also shows an alternate start site at aa 246.

• Long Name:

  SH2B adaptor protein 1

• Entrez Gene IDs:

  25970 (Human); 20399 (Mouse)

• Alternate Names:

  FLJ30542; KIAA1299; Pro-rich, PH and SH2 domain-containing signaling mediator; PSM; SH2 domain-containing protein 1B; SH2 domain-containing putative adapter SH2-B; SH2B adapter protein 1; SH2B adaptor protein 1; SH2-B signaling protein; SH2B1; SH2BDKFZp547G1110