• Species Reactivity

  Human

• Specificity

  Detects a 220 - 240 kDa cell-surface protein whose N-terminal amino acid sequence is identical to the rat NG2 chondroitin sulfate proteoglycan molecule (1).

• Source

  Monoclonal Mouse IgG    ₁ Clone # 7.1

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Human transformed stromal cells

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25 μg/10    ⁶ cells

  See below

• 
  

• Immunoprecipitation

  Smith, F.O.     et al. (1996) Blood.     87:1123. This application was not tested by R&D Systems.    
   

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NG2/MCSP

The clone 7.1 antibody recognizes a cell surface-expressed chondroitin sulfate proteoglycan that is the human homolog of rat NG2 antigen. The NG2 antigen, also known as chrondroitin sulfate proteoglycan 4, can be found on human glial cell population and is also expressed on myeloid and lymphoid cell subsets with chromosomal modifications.

• References:

1. Smith, F.O. et al. (1996) Blood. 87:1123.

• Long Name:

  Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan

• Entrez Gene IDs:

  1464 (Human); 121021 (Mouse); 81651 (Rat)

• Alternate Names:

  AN2; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan 4; CSPG4; EC 3.6.3; HMW-MAA; MCSP; MCSPChondroitin sulfate proteoglycan NG2; MCSPG; MCSPGMelanoma chondroitin sulfate proteoglycan; MEL-CSPG; MSK16Melanoma-associated chondroitin sulfate proteoglycan; NG2; NG2EC 2.7.8