• Species Reactivity

  Human

• Specificity

  Detects human CCL1/I?309/TCA?3 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CCL2, 3, 4, 5, 7, 8, 9/10/MIP-1 gamma, 11, 14, 17, 19, 20, 21, 25, 28, recombinant mouse CCL2, 3, 5, 6, 7, 9/10/MIP-1 gamma, 11, 21, or 25 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 35305

• Purification

  Protein A or G purified from ascites

• Immunogen

  E. coli-derived recombinant human CCL1/I?309/TCA?3    
  Lys24-Lys96    
  Accession # P22362

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  Recombinant Human CCL1/I?309/TCA?3 (Catalog # )

• 
  

• Human CCL1/I-309 Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 μg/mL 

  Human CCL1/I?309/TCA?3 Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 μg/mL 

  Human CCL1/I?309/TCA?3 Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Human CCL1/I-309 Protein (Catalog # )

• 
  

• Neutralization

  Measured by its ability to neutralize CCL1/I?309/TCA?3-induced chemotaxis in the BaF3 mouse pro?B cell line transfected with human CCR8. The Neutralization Dose (ND     ₅₀) is typically 0.1-0.5 μg/mL in the presence of 0.02 μg/mL Recombinant Human CCL1/I?309/TCA?3.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL1/I-309/TCA-3

Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a gamma /δ T cell line but not in EBV-transformed B cells. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Human CCL1 and mouse TCA3 also share significant sequence homology in the 5’ flanking region of their genes. CCL1 cDNA encodes a 96 amino acid precursor protein with a hydrophobic signal peptide that is cleaved to yield a 73 amino acid mature protein. The CCL1 sequence contains one potential N?linked glycosylation site, and natural CCL1 secreted by activated T cells is a glycoprotein doublet of 15?16 kDa. The amino acid sequence of CCL1 identified the protein as a member of the chemokine beta subfamily.

• References:

1. Miller, M.D. and M.S. Krangel (1992) Proc. Natl. Acad. Sci. USA 89:2950.

2. Miller, M.D. et al. (1990) J. Immunol. 145:2737.

• Entrez Gene IDs:

  6346 (Human); 20290 (Mouse)

• Alternate Names:

  C-C motif chemokine 1; CCL1; chemokine (C-C motif) ligand 1; I-309; inflammatory cytokine I-309; P500; SCYA1; SCYA1Small-inducible cytokine A1; SCYA2; SISe; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); T lymphocyte-secreted protein I-309; TCA3; TCA-3