• Species Reactivity

  Human, Mouse

• Specificity

  Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.

• Source

  Monoclonal Rat IgG    _2B Clone # 240408

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Oct?3/4    
  Met1-Asn265 (Met262Leu)    
  Accession # Q01860

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Fluorescein

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  10 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Oct-3/4

Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells.(2, 3) Oct-3/4 is required to sustain stem cell self-renewal and pluripotency.(4) It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.

• References:

1. Takeda, J.         et al. (1992) Nucleic Acids Res.         20:4613.

2. Scholer, H.R.         et al. (1989) EMBO J.         8:2543.

3. Rosner, M.H.         et al. (1990) Nature         345:686.

4. Niwa, H. et al. (2000) Nat. Genet. 24:372.

• Long Name:

  Octamer-binding Protein 3/4

• Entrez Gene IDs:

  5460 (Human); 18999 (Mouse)

• Alternate Names:

  3-Oct; Oct-3/4; Oct4; Otf3g; Otf4; Pou5f1