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詳細說明
概述
產(chǎn)品名稱Anti-HAPLN1抗體[EPR6338]
描述
兔單克隆抗體[EPR6338] to HAPLN1
經(jīng)測試應用WB,IHC-P,ICC/IF
種屬反應性
與反應: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) aa 300 to the C-terminus. The exact sequence is proprietary.
Database link: P10915陽性對照
Human spleen, MCF7 and Caco2 lysates; Human transitional cell carcinoma of bladder tissue; MCF7 cells.
常規(guī)說明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb? technology. RabMAb? technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存儲溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA純度Protein A purified
克隆單克隆
克隆編號EPR6338
同種型IgG
研究領域
Signal Transduction
Cytoskeleton / ECM
Extracellular Matrix
ECM Proteins
Other ECM Proteins
Anti-HAPLN1 antibody [EPR6338] 圖像
Immunocytochemistry/ Immunofluorescence - (ab181997)
Immunofluorescent analysis of MCF7 cells (paraformaldehyde-fixed, 4%) labeling HAPLN1 with ab181997 at 1/250 dilution, followed by Goat anti rabbit IgG(Dylight 488) at 1/200 dilution and counter-stained with DAPI (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab181997)
Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling HAPLN1 with ab181997 at 1/50 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Western blot - (ab181997)
All lanes : Anti-HAPLN1 antibody [EPR6338] (ab181997) at 1/1000 dilution
Lane 1 : MCF7 lysate
Lane 2 : Caco2 lysate
Lysates/proteins at 10 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L)-HRP at 1/1000 dilution
Predicted band size : 40 kDa
Western blot - (ab181997)
Anti-HAPLN1 antibody [EPR6338] (ab181997) at 1/1000 dilution + Human spleen lysate at 10 μg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/1000 dilution
Predicted band size : 40 kDa
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