詳細(xì)說明
| Replacement Information |
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| Description | |
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| Catalogue Number | 14-784 |
| Brand Family | Upstate |
| Trade Name |
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| Description | mTOR Protein and FKBP12 Protein, 1 kit |
| Overview | This product includes 10 μg of active mTOR (1362-end) and 1 mg of FKBP12. |
| Background Information | FKBP12: N-terminal 6His-tagged recombinant, full length, human FKBP12, expressed in E.coli. mTOR (1362-end), active: N-terminal FLAG?-tagged, recombinant, human mTOR, amino acids 1362-end, expressed by baculovirus in Sf21 insect cells. |
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| Product Information | |
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| Components |
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| Presentation | mTOR Enzyme in 10mM HEPES, 50mM NaCl, 50mM β-glycerophosphate, 0.25 mM sodium orthovanadate, 10 mM NaF, 300 μg/ml FLAG? peptide, 20% v/v glycerol. FKBP12in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Applications | |
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| Application | This product includes 10 μg of active mTOR & 1 mg of FKBP12 (mTOR: N-terminal FLAG-tagged, recombinant, human, amino acids 1362-end. FKBP12: N-terminal 6His-tagged recombinant, human, full length). |
| Biological Information | |
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| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The CDT6 gene is located in an intron of this gene. |
| Gene Symbol |
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| Protein Target | mTOR (FRAP1) |
| Target Sub-Family | Lipid/Atypical |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P42345 # Acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization. SIZE: 2549 amino acids; 288892 Da SUBUNIT: Interacts with the FKBP12-rapamycin complex. Binds UBQLN1. Forms part of the mammalian target of rapamycin 2 complex (TORC2) comprised of FRAP1, GBL, PRR5, RICTOR and SIN. TORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to PRR5 and RICTOR within the TORC2 complex. TISSUE SPECIFICITY: Expressed in numerous tissues, with highest levels in testis. SIMILARITY: SwissProt: P42345 ## Belongs to the PI3/PI4-kinase family. & Contains 1 FAT domain. & Contains 1 FATC domain. & Contains 7 HEAT repeats. & Contains 1 PI3K/PI4K domain. |
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| Storage Conditions | Store -70°C |
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| Material Size | 1 kit |
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