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T-Cell & B-Cell Activation miScript miRNA PCR Array

  T細胞和B細胞活化miRNA 芯片
 

ProductSpeciesTechnologyCat. No.T-Cell & B-Cell Activation miScript miRNA PCR ArrayHumanmiRNA ExpressionMIHS-111ZT-Cell & B-Cell Activation miScript miRNA PCR ArrayMousemiRNA ExpressionMIMM-111ZT-Cell & B-Cell Activation miScript miRNA PCR ArrayRatmiRNA ExpressionMIRN-111Z

The Human T-Cell & B-Cell Activation miScript miRNA PCR Array profiles the expression of 84 miRNAs involved in the activation and differentiation of T cells and B cells. This array provides scientific researchers with a convenient way to quickly analyze the miRNAs most relevant to specific stages of T cell and B cell development. Hematopoietic stem cells differentiate into lymphoid and myeloid progenitor cells. To develop into T cells, lymphoid progenitor cells migrate to the thymus, where T cell lineage commitment starts with the double negative (CD4-/CD8-) stage. The cells proceed through the double positive (CD4+/CD8+) stage, and end with the single positive stage, generating either na?ve CD4+ or na?ve CD8+ T cells. During the adaptive immune response, na?ve CD8+ T cells interact with antigen and activate, differentiating into effector T cells and potentially memory T cells after antigen clearance, whilena?ve CD4+ T cells activated by antigen differentiate into helper T cells. B cells mature in the spleen and remain na?ve until they encounter antigen. Activated B cells differentiate into plasma B cells or memory B cells, or undergo an intermediate differentiation step and switch antibody classes to become germinal center B cells. T cell and B cell activation involves many sequential steps and the careful timing of cellular signaling pathways. Specific miRNAs play roles in fine-tuning the differentiation and activation of these essential immune cells, and dysregulation of B cell or T cell functions can cause immunodeficiencies or cancer.A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR Green-based real-time PCR, the expression of a focused panel of miRNAs involved in T cell and B cell activation can be easily and reliably analyzed with this miScript miRNA PCR Array.
miScript miRNA PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
    T細胞和B細胞活化miRNA 芯片用于研究參與T細胞和B細胞的活化和分化的84 miRNA的表達。這個芯片為科研人員提供了一個方便快速的方法來分析特定階段T細胞和B細胞發(fā)育最相關的miRNA。造血干細胞分化成淋巴和髓系祖細胞。淋巴祖細胞遷移到胸腺, 保證T細胞譜系從雙陰性(CD4-/CD8-)階段開始，發(fā)展成T細胞。通過雙陽性細胞(CD4 + / CD8 +)階段,以單一陽性階段結束,生成未分化T細胞CD4 +或CD8 +。在適應性免疫反應過程, CD8 + T細胞與抗原相互作用和激活,分化成效應T細胞和潛在的記憶T細胞,而CD4 + T細胞被抗原激活分化成輔助T細胞。B細胞在脾中成熟并保持未分化狀態(tài),直到它們遇到抗原。激活B細胞分化成血漿B細胞或記憶B細胞,或經過一個中間分化的步驟和轉換抗體類型成為生發(fā)中心B細胞。T細胞和B細胞的激活涉及一序列的步驟和仔細的校時信號通路。特定的miRNA在微調重要免疫細胞的分化和激活中扮演重要角色,B細胞或T細胞功能的失調可以導致免疫缺陷或癌癥。張芯片含一個對照組使得分析數(shù)據(jù)時可以用相對定量ΔΔCT方法評估逆轉錄效率,基因組DNA污染,和PCR效率。利用這張芯片，通過實時定量PCR，可以簡易且可靠地檢測參與T細胞和B細胞的激活的miRNA的表達。
miRNA PCR芯片用于分子生物學應用。本產品不用于疾病的診斷、預防和治療。

T-Cell Differentiation*:
Double Negative (CD4-/CD8-):let-7d-5p, let-7e-5p, miR-126-3p, miR-128, miR-146b-5p, miR-15a-5p, miR-17-5p, miR-17-3p, miR-181c-5p, miR-191-5p, miR-19a-3p, miR-199a-5p, miR-20a-5p, miR-20b-5p, miR-221-3p, miR-222-3p, miR-223-3p, miR-28-5p, miR-29c-3p, miR-30e-5p, miR-342-3p, miR-423-5p, miR-93-5p, miR-98-5p.
Double Positive (CD4+/CD8+):let-7b-5p, miR-181a-5p, miR-181b-5p, miR-181d, miR-19b-3p.
CD4+ Na?ve:miR-132-3p, miR-146a-5p, miR-182-5p, miR-184, miR-25-3p, miR-326, miR-92a-3p.
CD8+ Na?ve:let-7a-5p, let-7c, let-7f-5p, let-7g-5p, miR-130b-3p, miR-139-5p, miR-142-3p, miR-150-5p, miR-155-5p, miR-15a-3p, miR-16-5p, miR-26a-5p, miR-26b-5p, miR-29b-3p, miR-30b-5p, miR-30c-5p, miR-30d-5p.
CD8+ Effector:miR-147a, miR-148a-3p, miR-18a-5p, miR-27a-3p, miR-27b-3p.
CD8+ Memory:let-7i-5p, miR-106b-5p, miR-142-5p, miR-15b-5p, miR-17-5p, miR-21-5p, miR-23a-3p, miR-23b-3p, miR-24-3p, miR-29a-3p, miR-31-5p.
B-Cell Differentiation*:
Na?ve:let-7a-5p, let-7b-5p, let-7d-5p, let-7g-5p, let-7i-5p, miR-101-3p, miR-132-3p, miR-142-3p, miR-142-5p, miR-150-5p, miR-181c-5p, miR-195-5p, miR-204-5p, miR-214-3p, miR-221-3p, miR-222-3p, miR-223-3p, miR-29b-3p, miR-30e-5p, miR-331-3p, miR-92a-3p.
Germinal Center:miR-106b-5p, miR-130b-3p, miR-132-3p, miR-148a-3p, miR-15a-5p, miR-15b-5p, miR-16-5p, miR-17-5p, miR-17-3p, miR-181a-5p, miR-181b-5p, miR-191-5p, miR-19a-3p, miR-19b-3p, miR-210, miR-23b-3p, miR-25-3p, miR-28-5p, miR-30d-5p, miR-93-5p, miR-98-5p.
Memory:miR-100-5p, miR-125b-5p, miR-145-5p, miR-146a-5p, miR-155-5p, miR-21-5p, miR-23a-3p, miR-24-3p, miR-26a-5p, miR-26b-5p, miR-27a-3p, miR-27b-3p, miR-29a-3p, miR-29c-3p, miR-30b-5p, miR-30c-5p, miR-34a-5p.
Differentially Expressed in Tregs:miR-100-5p, miR-125b-5p, miR-146a-5p, miR-181c-5p, miR-20b-5p, miR-21-5p, miR-31-5p, miR-335-5p, miR-365a-3p, miR-99a-5p.
T-Cell Activation:let-7e-5p, let-7g-5p, let-7i-5p, miR-106b-5p, miR-139-5p, miR-142-5p, miR-146a-5p, miR-146b-5p, miR-155-5p, miR-15a-5p, miR-15a-3p, miR-181a-5p, miR-181c-5p, miR-195-5p, miR-20b-5p, miR-214-3p, miR-223-3p, miR-23a-3p, miR-23b-3p, miR-25-3p, miR-26a-5p, miR-26b-5p, miR-27a-3p, miR-27b-3p, miR-29a-3p, miR-29b-3p, miR-29c-3p, miR-30a-5p, miR-30b-5p, miR-30e-5p, miR-342-3p, miR-346, miR-574-3p, miR-98-5p.
 

What miScript miRNA PCR Arrays offer?
   Guaranteed Performance* - ready-to-use for gene expression analysis
   Time & Cost Saving - less than 30 min hands-on time to analyze 84 or 372 miRNA
   Simple Data Analysis - easy-to-use Excel-Based Data Analysis Template 

T和B細胞活化miRNA 芯片,T細胞和B細胞活化miRNA 芯片T-Cell & B-Cell Activation miScript miRNA PCR Array

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