• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie? Blue stain.

• Activity

  Recombinant Human His6-alpha -Synuclein is ideal for use as a control substrate for in vitro Ubiquitin conjugation using select Ubiquitin E3 ligases such as CHIP/Stub1. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-alpha -Synuclein concentration of 0.5-2.5 μM.

• Source

  E. coli-derived Contains a C-terminal 6-His tag

• Accession #

• Predicted Molecular Mass

  15 kDa

Background: alpha-Synuclein

alpha -Synuclein is member of a family of small soluble proteins that include also beta -, and gamma -Synuclein. It is predominantly expressed in neurons of the central nervous system in the presynaptic region of nerve terminals, where cycles between free partially unfolded and helical membrane-bound forms.  alpha -Synuclein can self-aggregate in vivo and in vitro, forming various oligomeric species and fibrillar and amorphous aggregates. The fibrils and amyloidal forms of alpha -Synuclein are major components of Lewy bodies and Lewy neurites and have been linked to the pathogenesis of Parkinson’s disease, Parkinson’s disease dementia, and dementia with Lewy bodies. alpha -Synuclein aggregates can be also found associated with amyloid plaques in Alzheirmer’s disease.

• References:

1. Breydo L, Wu J.W., Uversky V.N. (2012)         Biochim Biophys Acta.         1822: 261

2. Chen R.H., et al. (2013)         J Biol Chem.         288: 7438

3. Li X., et al. (2008)         Acta Biochim Biophys Sin (Shanghai)         40: 406

4. Surguchov A. (2008)         Int Rev Cell Mol Biol         270: 225

5. Xia Q., et al. (2008)         Front Biosci.         13: 3850

• Entrez Gene IDs:

  6622 (Human); 20617 (Mouse); 29219 (Rat); 641350 (Porcine); 282857 (Bovine)

• Alternate Names:

  alpha-Synuclein; Lewy body) 4; MGC110988; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; non-A4 component of amyloid; PARK1; PARK4; PD1; SNCA; synuclein, alpha (non A4 component of amyloid precursor); Synuclein-alpha