• Purity

  >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Recombinant Human AICL/CLEC-2B Fc Chimera is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Human Galectin-1 (Catalog # 1152-GA) that produces 50% optimal binding response is 0.6-3 μg/mL.

• Source

  Chinese Hamster Ovary cell line, CHO-derived

  MD	Human IgG1 (Pro100-Lys330)	IEGR	Human AICL/CLEC-2B (Lys26-His149) with a Met79Thr substitution Accession # Q92478
  N-terminus			C-terminus

• N-terminal Sequence    
  Analysis

  Met

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  41 kDa

• SDS-PAGE

  48-65 kDa, reducing conditions

Data Images

Background: AICL/CLEC-2B

Activation-induced C-type lectin (AICL), also known as CLEC2B, is a 30-35 kDa variably glycosylated type-2 transmembrane member of the C-type lectin-like receptor (CTLR) family. AICL belongs to the subgroup of CLEC2 proteins that also includes CLEC2A/KACL, CLEC2D/LLT, and CD69/CLEC2C, all of which are encoded by the natural killer gene complex (1). Human AICL contains a single C-type lectin domain in its extracellular region and a 7 amino acid cytoplasmic tail (2). AICL is expressed on monocytes, macrophages, and granulocytes (3), and it is upregulated on TLR-activated monocytes and IL-12 + IL-18 activated NK cells (3, 4). AICL is an activating receptor that triggers TNF production by monocytes (3). It binds to NKp80 on NK cells, resulting in NK cell mediated lysis of the AICL expressing monocyte (3). In addition, the AICL-NKp80 axis mediates interactions between activated and resting NK cells (4).

• References:

1. Li, Y. et al. (2014) Front. Immunol. 5:123.

2. Hamann, J. et al. (1997) Immunogenetics 45:295.

3. Welte, S. et al. (2006) Nat. Immunol. 7:1334.

4. Klimosch, S.N. et al. (2013) Blood 122:2380.

• Long Name:

  Activation-Induced C-Type Lectin

• Entrez Gene IDs:

  9976 (Human)

• Alternate Names:

  Activation-induced C-type lectin; AICL; AICLC-type lectin superfamily member 2; CLEC2B; CLEC-2B; CLECSF2; CLECSF2IFN-alpha-2b-inducing-related protein 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 2 (activation-induced); C-type lectin domain family 2 member B; C-type lectin domain family 2, member B; HP10085; IFN-alpha2b-inducing related protein 1; IFNRG1