• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Recombinant Human LILRA2/CD85h/ILT1 is coated at 2 μg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7. (Catalog # ) binds with a typical ED    ₅₀ of 150-900 ng/mL.

• Source

  Human embryonic kidney cell, HEK293-derived Gly24-Asn449, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Gly24

• Predicted Molecular Mass

  48 kDa

• SDS-PAGE

  70-81 kDa, reducing conditions

Data Images

Background: LILRA2/CD85h/ILT1

LILRA2, also known as ILT1, CD85h, and LIR7, is an approximately 70 kDa variably glycosylated transmembrane protein that regulates immune cell activation (1). Mature human LILRA2 consists of a 426 amino acid (aa) extracellular domain (ECD) with 4 Ig-like domains, a 21 aa transmembrane segment, and a 13 aa cytoplasmic tail (2). Alternative splicing generates isoforms with short deletions between the fourth Ig-like domain and the transmembrane region, and an isoform that is truncated C-terminal to the fourth Ig-like domain (3, 4). LILRA2 is expressed on monocytes, neutrophils, basophils, and eosinophils (5-7). It contains a positively charged  arginine residue in its transmembrane segment, enabling association with the signaling protein Fc epsilon RI gamma (5). Cross-linking of LILRA2 on monocytes induces the production of multiple cytokines as well as the upregulation of Fc gamma receptors (6, 7). Cross-linking also restricts monocyte differentiation into immature dendritic cells, phagocytic activity, and antigen presentation to T cells (6, 7). R&D Systems in-house testing indicates that LILRA2 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (8).

• References:

1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.

2. Borges, L. et al. (1997) J. Immunol. 159:5192.

3. Jones, D.C. et al. (2009) Eur. J. Immunol. 39:3195.

4. Mamegano, K. et al. (2008) Genes Immun. 9:214.

5. Nakajima, H. et al. (1999) J. Immunol. 162:5.

6. Lu, H.K. et al. (2012) PLoS One 7:e33478.

7. Lee, D.J. et al. (2007) J. Immunol. 179:8128.

8. Zheng, J. et al. (2012) Nature 485:656.

• Long Name:

  Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 2

• Entrez Gene IDs:

  11027 (Human)

• Alternate Names:

  CD85 antigen-like family member H; CD85h antigen; CD85h; ILT1; ILT-1; ILT1CD85H; Leukocyte immunoglobulin-like receptor 7; leukocyte immunoglobulin-like receptor subfamily A member 2 soluble; leukocyte immunoglobulin-like receptor subfamily A member 2; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 2; LILRA2; LIR7; LIR7LIR-7Immunoglobulin-like transcript 1