• Purity

  >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Bioassay data are not available.

• Source

  Spodoptera frugiperda,     Sf 21 (baculovirus)-derived

  Mouse TROY (Glu30-Leu170) Accession # Q9JLL3	DIEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Glu30

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  42.3 kDa (monomer)

• SDS-PAGE

  45-50 kDa, reducing conditions

Background: TROY/TNFRSF19

TROY (also known as TAJ and TNFRSF19) is a 45?55 kDa member of the TNF receptor superfamily of molecules (1?4). It is widely expressed in the embryo, but shows limited expression in adult. In particular, embryonicis found in embryonic epithelium, neural tube, and mesenchyme, and developing neurons of the hippocampus and thalamus (5, 6). In the adult,is found in the nervous system associated with dorsal root and retinal ganglia neurons, astrocytes, and microglia, and in hair follicles where it is expressed by dermal papilla matrix cells (4, 7, 8, 9). In human,has also been found on melanoma cells, not normal melanocytes (10). is reportedly a component of the Nogo-A receptor, taking the place of p75 in a membrane-bound LINGO-1:NgR1:complex (6, 8, 9). This complex purportedly mediates axon repulsion initiated by one of five myelin-associated inhibitory factors (OMpg; CD100; Ephrin B3; Nogo-A; MAG) (6, 11). In the hair follicle,binds LTa and acts with Eda to drive secondary hair follicle development (4, 12). Mature humanis a 394 amino acid (aa) type I transmembrane protein (1-3). It possesses a 141 aa extracellular region (aa 30-170) that contains three 40 aa TNFR cysteine motifs, and an extended 232 aa cytoplasmic domain (aa 192-423). There is one potential isoform that shows a two aa substitution for aa 416-423. The extracellular domains of human and mouseshare 90% aa identity.

• References:

1. Hu, S.         et al. (1999) Genomics         62:103.

2. Eby, M.T.         et al. (2000) J. Biol. Chem.         275:15336.

3. Kojima, T.         et al. (2000) J. Biol. Chem.         275:20742.

4. Hashimoto, T.         et al. (2008) Cell Cycle         7:106.

5. Pispa, J.         et al. (2003) Gene Exp. Patterns         3:675.

6. Mi, S. (2008) Cytokine Growth Factor Rev.         19:245.

7. Satoh, J.         et al. (2007) Neuropathol. Appl. Neurobiol.         33:99.

8. Shao, Z.         et al. (2005) Neuron         45:353.

9. Park, J.B.         et al. (2000) Neuron         45:345.

10. Spanjaard, R.A.         et al. (2007) Int. J. Cancer         120:1304.

11. Venkatesh, K.         et al. (2007) J. Cell Biol.         177:393.

12. Pispa, J. et al. (2008) Hum. Mol. Genet. 17:3380.

• Long Name:

  Tumor Necrosis Factor Receptor Superfamily, Member 19

• Entrez Gene IDs:

  55504 (Human); 29820 (Mouse)

• Alternate Names:

  TAJ; TAJ-alpha; TAJToxicity and JNK inducer; TNFRSF19; TRADE; TROY; TROYtumor necrosis factor receptor superfamily member 19; tumor necrosis factor receptor superfamily, member 19