• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Recombinant Viral EBOV GP is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human CLEC10A/CD301 (Catalog # ) that produces 50% of the optimal binding response is approximately 20-100 ng/mL.

• Source

  Chinese Hamster Ovary cell line, CHO-derived

  Z. ebolavirus GP1 (Ile33-Arg501) Accession # Q05320
  Z. ebolavirus GP2 (Glu502-Gln650) Accession # Q05320	HHHHHH
  N-terminus	C-terminus

• N-terminal Sequence    
  Analysis

  Ile33 (GP1) & Glu502 (GP2)

• Structure / Form

  Disulfide-linked heterodimer

• Predicted Molecular Mass

  69 kDa

• SDS-PAGE

  17-24 kDa and 105-120 kDa, reducing conditions

Background: EBOV GP

The GP glycoprotein encoded by the genome of Ebola family viruses is a critical molecule for the pathogenicity of   Ebolavirus hemorrhagic viruses (1, 2). It is processed into distinct forms for virus capsule or cell surface presentation or release from virus infected cells. The GP precursor protein is cleaved by furin at a multibasic site to yield a 140 kDa N-terminal fragment (GP1) and a 26 kDa C-terminal fragment (GP2) which remain disulfide linked (3). GP1 is entirely extracellular while GP2 is a transmembrane protein (4). Heterodimers of GP1-GP2 can further associate into trimers (5). GP expressed on virus infected cells can be shed by TACE mediated cleavage, liberating a disulfide linked complex of soluble GP1 and truncated GP2 (4-6). GP binds to multiple C-type lectins on target cell surfaces, including CLEC10A/MGL, DC-SIGN, and DC-SIGNR (7-9). Following internalization, GP1 is cleaved by Cathepsin B and Cathepsin L and then interacts with Niemann-Pick C1 (NPC1) in the endosomal membrane (10-12).

• References:

1. Yang, Z.-Y. et al. (2000) Nat. Med. 6:886.

2. de La Vega, M.-A. et al. (2015) Viral Immunol. 28:3.

3. Volchkov, V.E. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5762.

4. Volchkov, V.E. et al. (1998) Virology 245:110.

5. Sanchez, A. et al. (1998) J. Virol. 72:6442.

6. Dolnik, O. et al. (2004) EMBO J. 23:2175.

7. Takada, A. et al. (2004) J. Virol. 78:2943.

8. Alvarez, C.P. et al. (2002) J. Virol. 76:6841.

9. Simmons, G. et al. (2003) Virology 305:115.

10. Schornberg, K. et al. (2006) J. Virol. 80:4174.

11. Chandran, K. et al. (2005) Science 308:1643.

12. Cote, M. et al. (2011) Nature 477:344.

• Long Name:

  Ebola Virus Glycoprotein

• Entrez Gene IDs:

  911829 (Viral)

• Alternate Names:

  bovGP; EBOV GP