• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 μL droplet containing 200 ng on a nitrocellulose-coated microplate.

• Source

  Spodoptera frugiperda,     Sf 21 (baculovirus)-derived

  Rat Nogo-A (Arg1026-Leu1090) Accession #Q9JK11	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Arg1026

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  34.5 kDa (monomer)

Background: Nogo-A

Rat Nogo-A is a member of the reticulon family of transmembrane proteins. This family is characterized by the presence of a nonsignal sequence-containing N-terminus, a topologically conserved approximately 200 amino acid (aa) C-terminus that contains two transmembrane domains and an ER-retention motif, and a punctate intracellular distribution within the ER that is reminescent of a reticulum (1, 2). Nogo-A in rat exists in four isoforms (3 - 5). The full length rat Nogo-A is 1163 aa in length and contains a 989 aa N-terminus, a 21 aa transmembrane segment, a 94 aa connecting “l(fā)oop”, a second 21 aa transmembrane segment, and a 38 aa C-terminus. Three areas are of particular interest. One is a stretch of 66 aa within the 94 aa transmembrane connecting loop (SWISSPROT defines this region as being 94 aa in length while the original cloning papers identified it as being 66 aa in length). This segment is reported to bind to the GPI-linked Nogo receptor/p75 complex on axons and induce growth cone collapse (6 - 8). Two other areas in the N-terminus have also been discovered to have bioactivity (6, 9, 10). Amino acids 59 - 172 are reported to block fibroblast spreading, while aa 544 - 725 block neurite outgrowth and block fibroblast spreading (6, 10). The exact topology of Nogo-A is unclear. With two transmembrane segments, the N- and C-termini may be extracellular with the “l(fā)oop” region intracellular, or the situation could be reversed (11, 12). Alternatively, the loop region and N-terminus may be on the same side of the membrane (6). Nogo-A is expressed in neurons, endothelial cells. oligodendrocytes, fibroblasts and myoblasts (10, 13, 14). Rat Nogo-A is 78% aa identical to human Nogo-A overall, with 98% aa identical in the loop region and 81% aa identity in the 544 - 725 aa segment.

• References:

1. Oertle, T. et al. (2003) FASEB J. 17:1238.

2. GrandPre, T. et al. (2000) Nature 403:439.

3. Chen, M.S. et al. (2000) Nature 403:434.

4. Morris, N.J. et al. (1999) Biochim. Biophys. Acta 1450:68.

5. Ito, T. and S.M. Schwartz (1999) GenBank Accession # Q9JK11.

6. Oertle, T. et al. (2003) J. Neurosci. 23:5393.

7. Fournier, A.E. et al. (2001) Nature 409:341.

8. Wang, K.C. et al. (2002) Nature 420:74.

9. Prinjha, R. et al. (2000) Nature 403:384.

10. Dodd, D.A. et al. (2005) J. Biol. Chem. 280:12494.

11. Huber, A.B. and M.E. Schwab (2000) Biol. Chem. 381:407.

12. Ng, C.E.L. and B.L. Tang (2002) J. Neurosci. Res. 67:559.

13. Wang, X. et al. (2002) J. Neurosci. 22:5505.

14. Acevedo, L. et al. (2004) Nat. Med. 10:382.

• Long Name:

  Reticulon 4A

• Entrez Gene IDs:

  57142 (Human); 68585 (Mouse); 83765 (Rat)

• Alternate Names:

  NI220; NogoA; Nogo-A; RTN4; RTN4A