• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <1.0 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When 25 ng/mL of rrIL-7R alpha /Fc Chimera is captured by goat anti-mouse IgG Fc Chimera (Catalog # G-202-C) coated plate, the concentration of rmIL-7 that produces 50% optimal binding response is found to be approximately 10-30 ng/mL.

• Source

  Mouse myeloma cell line, NS0-derived

  Rat IL-7R alpha (Glu21 - Asp239) Accession # NP_001099888	IEGRMDP	Mouse IgG2a (Glu98 - Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Glu21

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  52.2 kDa (monomer)

• SDS-PAGE

  60-75 kDa under reducing conditions

Background: IL-7 R alpha/CD127

Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature rat IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type-III domain and a WSxWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3, Accession # NP_001099888). Within the ECD, rat IL-7 R alpha shares 67% and 79% aa sequence identity with human and mouse IL-7 R alpha, respectively. IL-7 R alpha associates with the common gamma chain ( gamma _c) to form the functional high affinity IL-7 receptor complex (4). The gamma _c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL-7 show cross-species activity through the IL-7 receptor (3, 5). IL-7 R alpha is expressed on double negative (CD4^-CD8^-) and CD4⁺ or CD8⁺ single positive T cells as well as on CD8⁺ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development (8). In human, by contrast, it is required for T cell but not for B cell development (9). IL-7 induces the downregulation and shedding of cell surface IL-7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).

• References:

1. Mazzucchelli, R. and S.K. Durum (2007) Nat. Rev. Immunol. 7:144.

2. Liu, Y.-J. et al. (2007) Annu. Rev. Immunol. 25:193.

3. Goodwin, R.G. et al. (1990) Cell 60:941.

4. Noguchi, M. et al. (1993) Science 262:1877.

5. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.

6. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125.

7. Kaech, S.M. et al. (2003) Nat. Immunol. 4:1191.

8. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.

9. Prieyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. 93:10348.

10. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.

11. Park, L.S. et al. (2000) J. Exp. Med. 192:659.

12. Pandey, A. et al. (2000) Nat. Immunol. 1:59.

13. Reche, P.A. et al. (2001) J. Immunol. 167:336.

14. Al-Shami, A. et al. (2004) J. Exp. Med. 200:159.

15. Jahn, T. et al. (2007) Blood 110:1840.

16. Lai, L. et al. (2006) Blood 107:1776.

• Long Name:

  Interleukin 7 Receptor alpha

• Entrez Gene IDs:

  3575 (Human); 16197 (Mouse); 294797 (Rat)

• Alternate Names:

  CD127 antigen; CD127; CD127ILRA; CDW127; IL-7 R alpha; IL-7 receptor subunit alpha; IL7R alpha; IL-7R subunit alpha; IL7R; IL7RA; IL-7Ra; IL-7R-alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; interleukin 7 receptor; interleukin-7 receptor subunit alpha