• Species Reactivity

  Human

• Specificity

  Detects human IL-22 in direct ELISAs. In direct ELISAs, this antibody shows 100% cross-reactivity with recombinant mouse IL?22 and recombinant rat IL?22 and no cross-reactivity with recombinant human IL?10.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 142928

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human IL-22    
  Ala34-Ile179    
  Accession # Q9GZX6

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  10 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-22

Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT1 and STAT3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22 R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

• References:

1. Dumoutier, L. et al. (2000) J. Immunol. 164:1814.

2. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.

3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.

4. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.

• Long Name:

  Interleukin 22

• Entrez Gene IDs:

  50616 (Human); 50929 (Mouse); 500836 (Rat)

• Alternate Names:

  Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL-21; IL22; IL-22; IL-22IL21; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 21; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18