• Purity

  >90%, by SDS-PAGE with silver staining

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When recombinant human Slit3 or Recombinant Mouse Slit3 (Catalog # ) is coated at 0.25 μg/mL, Recombinant Mouse ROBO2 Fc Chimera binds with an apparent K    _d <2 nM.  

• Source

  Mouse myeloma cell line, NS0-derived

  Mouse ROBO2 (Ser22-Ala863) Accession # NP_780758	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Ser22

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  120 kDa

• SDS-PAGE

  100-155 kDa, reducing conditions

Background: ROBO2

ROBO2 is a type I transmembrane glycoprotein that is part of the ROBO family of guidance molecules. Mouse ROBO2 is synthesized as a 1470 amino acid (aa) precursor and has a predicted molecular weight of approximately 160 kDa. Its extracellular domain contains five C2-type Ig-like domains and three fibronectin type III domains, and shares 98% aa sequence identity with the human and rat orthologs. Alternative splicing of mouse   Robo2 creates an 820 aa isoform that contains a 14 aa substitution for aa 807-946 and then terminates after this substitution. A 1527 aa isoform, termed ROBO2a, has also been identified (1). ROBO2a contains a longer signal peptide and is cleaved at a different position resulting in four extra aa in its N-terminus. It also contains an additional 42 aa that is inserted after aa 1049 (1). Mouse ROBO2 is ubiquitously expressed in embryonic and adult tissues, with the highest expression levels in the adult being seen in the brain, liver, and kidney (1-9). Mouse ROBO2a is also ubiquitously expressed during development; however, it is only expressed in the brain in adults (1). ROBO2 acts as a receptor for the Slit family of chemorepulsion molecules and facilitates Slit-mediated growth cone repulsion (5). It is highly expressed on axons that do not cross the ventral midline of the nervous system and on axons that have already crossed (9-11). As a result, Slit/ROBO2 signaling regulates guidance and midline crossing of growing axons (3, 5, 11-16). Slit/ROBO2 signaling has also been shown to mediate repulsion of neuronal cell bodies away from the floor plate during development (12). Furthermore, interactions between Slit and ROBO2 have also been shown to be critical for development of the foregut, lungs, kidney, and heart (6-8, 17, 18).

• References:

1. Yue, Y. et al. (2006) Genomics 88:772.

2. Piper, M. et al. (2000) Mech. Dev. 94:213.

3. Erskine, L. et al. (2000) J. Neurosci. 20:4975.

4. Vargesson, N. et al. (2001) Mech. Dev. 106:175.

5. Guthrie, S. (2001) Curr. Biol. 11:R300.

6. Greenberg, J.M. et al. (2004) Dev. Dyn. 230:350.

7. Medioni, C. et al. (2010) Dev. Dyn. 239:3303.

8. Domyan, E.T. et al. (2013) Dev. Cell 24:52.

9. Sundaresan, V. et al. (2004) J. Comp. Neurol. 468:467.

10. Rajagopalan, S. et al. (2000) Neuron 28:767.

11. Mambetisaeva, E.T. et al. (2005) Dev. Dyn. 233:41.

12. Kim, M. et al. (2011) Dev. Biol. 358:181.

13. Fouquet, C. et al. (2007) J. Neurosci. 27:3037.

14. López-Bendito, G. et al. (2007) J. Neurosci. 27:3395.

15. Goldberg, D. et al. (2013) Dev. Dyn. 242:9.

16. Wang, S. et al. (2013) J. Neurosci. 33:12242.

17. Grieshammer, U. et al. (2004) Dev. Cell 6:709.

18. Mommersteeg, M.T. et al. (2013) Circ. Res. 112:465.

• Long Name:

  Roundabout Homolog 2

• Entrez Gene IDs:

  6092 (Human); 268902 (Mouse); 84409 (Rat)

• Alternate Names:

  Drosophila) homolog 2; ROBO2; roundabout, axon guidance receptor, homolog 2 (Drosophila); SAX3