• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. Recombinant Human Osteoadherin, immobilized at 20 μg/mL (100 μL/well) can induce more than 35% of ATDC-5 cell adhesion.

• Source

  Mouse myeloma cell line, NS0-derived Gln21-Glu421, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  No results obtained: Gln21 predicted    
   

• Predicted Molecular Mass

  47.9 kDa

• SDS-PAGE

  60-66 kDa, reducing conditions

Background: Osteoadherin/OSAD

Osteoadherin (OSAD), also known as Osteomodulin, is an extracellular matrix keratan sulfate proteoglycan that belongs to the class II subfamily of small leucine-rich proteoglycans (SLRP). LRR motifs consist of approximately 20 - 30 amino acids (aa) with conserved leucine spacing, folded into a structure with one beta -sheet and one alpha -helix (1, 2). The human OSAD cDNA encodes a 421 aa precursor that contains a 20 aa signal sequence and eleven tandem leucine rich repeats (3). Human OSAD shares 80 - 84% aa sequence identity with bovine, mouse, and rat OSAD. Human OSAD shares 32 - 35% aa sequence identity with human class II SLRPs Fibromodulin, Keratocan, Lumican, and PRELP. Bovine, mouse, and rat OSAD are expressed as 60 - 85 kDa molecules, although the amino acid sequence for each predicts a size of 46 - 47 kDa. The primary difference is due to the presence of extensive N-linked glycosylation that can also vary between tissues of the same species (4, 5). Human OSAD is expressed as an even larger 110 kDa molecule in teeth (6). OSAD contains eight sulfated tyrosine residues (4, 7) and is distinguished from other class II SLRPs by the presence of an approximately 70 aa C-terminal acidic domain (3). OSAD is expressed by fetal and adult osteoblasts but is not detectable in cartilage or tendon (3, 4, 8). In dental tissue, OSAD is expressed by odontoblasts and ameloblasts (5, 9 - 11) and is involved in the mineralization of bone and teeth (5, 11, 12). OSAD promotes the adhesion of osteoblasts and odontoblasts to the surrounding matrix, an interaction that is mediated by Integrin alpha V beta 3 (4, 6).

• References:

1. Matsushima, N. et al. (2000) Proteins 38:210. 

2. Kobe, B. and A.V. Kajava (2001) Curr. Opin. Struct. Biol. 11:725. 

3. Sommarin, Y. et al. (1998) J. Biol. Chem. 273:16723. 

4. Wendel, M. et al. (1998) J. Cell Biol. 141:839. 

5. Hultenby, P.U. et al. (2003) Eur. J. Oral Sci. 111:128. 

6. Lucchini, M. et al. (2004) J. Dent. Res. 83:552. 

7. Onnerfjord, P. et al. (2004) J. Biol. Chem. 279:26

8. Shen, Z. et al. (1999) Matrix Biol. 18:533.

9. Buchaille, R. et al. (2000) Bone 27:265.

10. Buchaille, R. et al. (2000) Matrix Biol. 19:421.

11. Couble, M.L. et al. (2004) Histochem. Cell Biol. 121:47.

12. Ramstad, V.E. et al. (2003) Calcif. Tissue Int. 72:57.

• Entrez Gene IDs:

  4958 (Human)

• Alternate Names:

  Keratan sulfate proteoglycan osteomodulin; KSPG osteomodulin; OMD; OSAD; osteoadherin proteoglycan; Osteoadherin; osteomodulin; SLRR2C; SLRR2Costeoadherin