• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of HT?29 human colon adenocarcinoma cells. When 5 x 10    ⁴ cells/well are added to rhLAIR-2 coated plates (50 μg/mL with    
  100 μL/well), approximately 40%-60% will adhere after 10 minutes at 37° C.    
     Optimal concentration depends on cell type as well as the application or research objectives.  

• Source

  Mouse myeloma cell line, NS0-derived Gln22-Pro152, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  No results obtained: Gln22 predicted & Thr37

• Predicted Molecular Mass

  14.9 kDa

• SDS-PAGE

  20 kDa, reducing conditions

Background: LAIR2

LAIR-2 (leukocyte-associated Ig-like receptor-2; CD306) is a secreted, 131 amino acid (aa) protein that contains one Ig-like C2 type domain, making it a member of the Ig superfamily. When compared to LAIR-1, its transmembrane counterpart, it shares 83% aa identity across the signal sequence and extracellular domains; (1-3) although one is secreted and one is membrane-bound, the two LAIR proteins are thought to have arisen from a common gene ancestor and appear to share similar adhesion profiles. This suggests that LAIR-2 may compete with LAIR-1 for ligand binding (3, 4). A 114 aa alternate splice form of LAIR-2 is truncated at the C terminus, but retains the entire Ig domain (1-3). The expression profile of these splice forms, and the presence of orthologs in other species, have not been reported.

• References:

1. Meyaard, L. (2003) J. Biol. Regul. Homeost. Agents 17:330.

2. Meyaard, L. et al. (1999) J. Immunol. 162:5800.

3. Meyaard, L. et al. (1997)  Immunity 7:283.

4. Xu, X.G. et al. (2005) Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 21:553.

• Long Name:

  Leukocyte-associated Ig-like Receptor 2

• Entrez Gene IDs:

  3904 (Human)

• Alternate Names:

  CD306 antigen; CD306; CD306leukocyte-associated Ig-like receptor-2; LAIR2; LAIR-2; leukocyte-associated Ig-like receptor 2; leukocyte-associated immunoglobulin-like receptor 2; MGC71634