• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to enhance neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly enhance neurite outgrowth when immobilized at 3-6 μg/mL.

• Source

  Mouse myeloma cell line, NS0-derived Glu39-Val665, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Glu39

• Predicted Molecular Mass

  70.8 kDa

• SDS-PAGE

  100-120 kDa, reducing conditions

Background: SLITRK5

SLITRK5 (Slit and Trk-like family member 5; also named LRR-containing protein 11) is a member of the SLITRK family of proteins (1, 2). This family currently includes six members, all of which contain a slit-like extracellular region and (save for SLITRK1) a Trk-like cytoplasmic region (3). Human SLITRK5 is a type I transmembrane (TM) protein that is 958 amino acids (aa) in length. It contains a putative 40 aa signal sequence, a 624 aa extracellular domain (ECD), a 21 aa TM segment, and a 273 aa cytoplasmic region. The ECD is characterized by the presence of two leucine-rich domains (LRD) that resemble those found in slit. Each LRD (aa 80 - 285 plus aa 384 - 579) contains seven and eight leucine-rich repeats (LRRs), respectively. The exact function of the LRRs is not known. Based on other LRRs, it might be assumed that they mediate protein-protein-interaction (4). The cytoplasmic region contains two conserved, potential phosphorylation sites at Tyr 833 and Tyr 917, plus a TrkA-like tyrosine phosphorylation site (YLxxL) at Tyr 945. SLITRK5 is expressed on embryonic neurons of the hippocampus (pyramidal layer) and thalamus (3). Based on SLITRK2 studies, SLITRK5 will block neurite extension (3). Human SLITRK5 shares 97% and 98% aa identity with mouse and canine SLITRK5, respectively.

• References:

1. Aruga, J. et al. (2003) Gene 315:87.

2. Nagase, T. et al. (2001) DNA Res. 8:179.

3. Aruga, J. and K. Mikoshiba (2003) Mol. Cell. Neurosci. 24:117.

4. Enkhbayar, P. et al. (2003) Proteins 54:394.

• Long Name:

  SLIT and NTRK-like Family, Member 5

• Entrez Gene IDs:

  26050 (Human); 75409 (Mouse); 306152 (Rat)

• Alternate Names:

  bA364G4.2; FLJ58374; KIAA0918; leucine-rich repeat-containing protein 11; LRRC11; SLIT and NTRK-like family, member 5; SLIT and NTRK-like protein 5; SLITRK5