• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of Neuro?2A mouse neuroblastoma cells. The ED    ₅₀ for this efffect is 1.0-4.0 μg/mL.    
     Optimal dilutions should be determined by each laboratory for each application.  

• Source

  Chinese Hamster Ovary cell line, CHO-derived

  Human Contactin-6 (Asp20-Ser891) Accession # Q9UQ52	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Asp20

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  122.9 kDa

• SDS-PAGE

  130-170 kDa, reducing conditions

Background: Contactin-6

Contactin-6, also known as NB-3, is a 130 kDa member of the TAG/F3 subgroup of Contactin GPI?linked neuronal adhesion proteins (1, 2). Mature human Contactin-6 consists of six immunoglobulin?like domains (aa 26?587), a flexible linker region, four fibronectin type III domains (aa 597?992), and a GPI anchor (3). Within aa 20?891, human Contactin-6 shares 89% aa sequence identity with mouse and rat Contactin-6. Contactin-6 expression is upregulated postnatally in the cerebellum, brainstem, and hippocampus, and it shows differential expression within various structures of the brain (3?7). In the cerebellum, it is presynaptically localized at synapses between excitatory glutamatergic parallel fibers and Purkinje cells (7). Contactin-6 associates   in cis with CHL-1, PTPR alpha, and PTPR gamma as well as   in trans with Notch-1 on oligodendrocytes (6, 8?10). Its binding to Notch-1 triggers the release and translocation of the Notch intracellular domain to the nucleus and promotes oligodendrogenesis (6). Contactin-6 function is important in the postnatal development and function of the cerebellum, as shown in knockout mice which exhibit poor motor coordination (5, 7).

• References:

1. Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.

2. Stoeckli, E.T. (2010) Cell Adh. Migr. 4:523.

3. Kamei, Y. et al. (1998) J. Neurosci. Res. 51:275.

4. Lee, S. et al. (2000) Gene 245:253.

5. Takeda, Y. et al. (2003) J. Neurobiol. 56:252.

6. Cui, X.-Y. et al. (2004) J. Biol. Chem. 279:25858.

7. Sakurai, K. et al. (2009) Dev. Neurobiol. 69:811.

8. Ye, H. et al. (2008) EMBO J. 27:188.

9. Bouyain, S. and D.J. Watkins (2010) Proc. Natl. Acad. Sci. 107:2443.

10. Ye, H. et al. (2011) J. Biol. Chem. 286:26071.

• Entrez Gene IDs:

  27255 (Human); 53870 (Mouse); 27256 (Rat)

• Alternate Names:

  CNTN6; contactin 6; Contactin6; Contactin-6; MGC133256; NB3