• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to induce CXCL10 secretion by RAW 264.7 mouse monocyte/macrophage cells. The ED    ₅₀ for this effect is 1.5-7.5 μg/mL.

• Source

  Mouse myeloma cell line, NS0-derived Asn16-Pro333, with an N-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  His

• Predicted Molecular Mass

  34 kDa

• SDS-PAGE

  36-40 kDa, reducing conditions

Data Images

Background: CTRP9/C1qTNF9

C1qTNF9, also known as CTRP9, is an approximately 40 kDa member of the C1q and TNF-related protein family (1). Like all members of this protein family, C1qTNF9 consists of a short variable region, a collagenous domain that can be hydroxylated, and a C1q-like globular domain (1). Human C1qTNF9 shares 85% amino acid sequence identity with the mouse and rat orthologs. Both the mouse and human C1qTNF9 proteins are expressed in adipose tissue, but the mouse protein has also been detected in the heart, lung, muscle, kidney, testis, lymph node, smooth muscle, prostate, thymus, and uterus (1, 2). They have both also been shown to be secreted as trimers and higher order multimers and also to form hetero-oligomers with Adiponectin (1, 2). Mouse C1qTNF9 can stimulate the phosphorylation of AMPK, Akt, and eNOS (1, 3, 4). Also in mice, C1qTNF9 may have an important role in cardiac and metabolic health. Its expression has a cardioprotective effect following acute myocardial infarction that may be dependent on AMPK activation (4-6). Additionally, transgenic mice overexpressing C1qTNF9 are resistant to high fat diet?induced obesity (7). This metabolic role may be conserved, since C1qTNF9 serum levels have been shown to inversely correlate with metabolic syndrome in humans (8).

• References:

1. Wong, G.W. et al. (2009) FASEB J. 23:241.

2. Peterson, J.M. et al. (2009) Biochem. Biophys. Res. Commun. 388:360.

3. Zheng, Q. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:2616.

4. Kambara, T. et al. (2012) J. Biol. Chem. 287:18965.

5. Su, H. et al. (2013) Basic Res. Cardiol. 108:315.

6. Sun, Y. et al. (2013) Circulation 128:S113.

7. Peterson, J.M. et al. (2013) Am. J. Physiol. Regul. Integr. Comp. Physiol. 305:R522.

8. Hwang, Y.C. et al. (2013) Int. J. Obes. (Lond) [Epub ahead of print].

• Long Name:

  C1q and Tumor Necrosis Factor Related Protein 9

• Entrez Gene IDs:

  338872 (Human); 239126 (Mouse)

• Alternate Names:

  AQL1; C1q and tumor necrosis factor related protein 9; C1qTNF9; C1QTNF9AComplement C1q tumor necrosis factor-related protein 9; complement C1q tumor necrosis factor-related protein 9A; CTRP9; MGC48915