• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Recombinant Human Neuroligin 2/NLGN2 is immobilized at 1.5 μg/mL, Recombinant Human Neurexin 1 beta /NXRN1b Fc Chimera (Catalog # ) binds with an apparent K    _d <10 nM.  

• Source

  Mouse myeloma cell line, NS0-derived Gln15-Ser660, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  No results obtained: Gln15 predicted

• Predicted Molecular Mass

  72 kDa

• SDS-PAGE

  85-95 kDa, reducing conditions

Background: Neuroligin 2/NLGN2

Neuroligin 2 (NLGN2) is one of several type I transmembrane Neuroligins that are expressed on neuronal postsynaptic densities. Neuroligins play an important role in synaptic development and function (1). Mature human Neuroligin 2 is a 105 kDa protein that consists of a 663 amino acid (aa) extracellular domain (ECD) with a catalytically inactive cholinesterase-like domain, a 21 aa transmembrane segment, and a 137 aa cytoplasmic tail (2, 3). Within the ECD, human Neuroligin 2 shares 98% aa sequence identity with mouse and rat Neuroligin 2. Alternate splicing generates an isoform with a 17 aa insertion at splice site A within the ECD (2). This recombinant protein does not contain the splice site A insert (-SS A). Neuroligin 2 is expressed on neurons in the brain and also on pancreatic beta  cells where it facilitates insulin secretion (2, 4, 5). The -SS A isoform is uniformly expressed among inhibitory and excitatory synapses, while the +SS A isoform is enriched at inhibitory GABAergic synapses (4, 6, 7). Neuroligin 2 clusters at postsynaptic densities in association with other postsynaptic proteins including S-SCAM, PSD-95, gephyrin, and Neuroligin 3 (4, 8-10). Synaptic maturation is promoted by the binding of Neuroligin 2 with presynaptic Neurexins, and these interactions are restricted to particular combinations of isoforms of the binding partners (11-15). Neuroligin 2 interacts with the alpha and beta forms of Neurexin 1, 2, and 3 (14). Its -SS A and +SS A isoforms are bound equally well by Neurexin 1 beta isoforms (-SS4 or +SS4), although only the Neurexin 1 beta +SS4 isoform can induce development of Neuroligin 2-dependent GABAergic contacts (7, 15).

• References:

1. Sudhof, T.C. (2008) Nature 455:903.

2. Ichtchenko, K. et al. (1996) J. Biol. Chem. 271:2676.

3. Koehnke, J. et al. (2008) Proc. Natl. Acad. Sci. 105:1873.

4. Varoqueaux, F. et al. (2004) Eur. J. Cell Biol. 83:449.

5. Suckow, A.T. et al. (2008) Endocrinology 149:6006.

6. Graf, E.R. et al. (2004) Cell 119:1013.

7. Chih, B. et al. (2006) Neuron 51:171.

8. Sumita, K. et al. (2007) J. Neurochem. 100:154.

9. Irie, M. et al. (1997) Science 277:1511.

10. Budreck, E.C. and P. Scheiffele (2008) Eur. J. Neurosci. 26:1738.

11. Chih, B. et al. (2005) Science 307:1324.

12. Chubykin, A.A. et al. (2007) Neuron 54:919.

13. Hines, R.M. et al. (2008) J. Neurosci. 28:6055.

14. Kang, Y. et al. (2008) J. Biol. Chem. 283:2323.

15. Graf, E.R. et al. (2006) J. Neurosci. 26:4256.

• Entrez Gene IDs:

  57555 (Human); 216856 (Mouse); 117096 (Rat)

• Alternate Names:

  EC 3.1.1; EC 3.1.1.1; KIAA1366neuroligin-2; Neuroligin 2; NLGN2