• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of HEK293T human embryonic kidney cells.

  When 5 x 10⁴ cells per well are added to human Olfactomedin-2 coated plates (1 μg/mL, 100 μL/well), approximately 50%-75% will adhere after 1 hour at 37 °C.

  Optimal dilutions should be determined by each laboratory for each application.

• Source

  Chinese Hamster Ovary cell line, CHO-derived Gln21-Pro454, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  No results obtained: Gln21 predicted

• Predicted Molecular Mass

  50 kDa

• SDS-PAGE

  55-75 kDa, reducing conditions

Background: Olfactomedin-2/Noelin-2

Olfactomedin-2 (OLFM2, OM2), also called OlfC or neuronal olfactomedin-related endoplasmic reticulum-localized-2 (Noelin-2) is a 50 kDa (predicted), secreted member of the olfactomedin/noelin family of proteins (1 - 3). Since Noelin-2 has also been used to designate an alternately spliced form of Noelin-1, Olfactomedin-2 is the less ambiguous name (4). Olfactomedin domain-containing proteins are often found in neural tissues (3). Human Olfactomedin-2 expressed sequence tags have mainly been found in the brain and eye, and RNA in the cerebellum (1, 2). In zebrafish, Olfactomedin-2 expression is developmentally regulated and, like Noelin-1, may play a role in neural crest cell differentiation (5). The human Olfactomedin-2 cDNA encodes a 20 amino acid (aa) signal sequence and a 454 mature protein that contains two coiled-coil regions (aa 58 - 85 and 136 - 193) and an Olfactomedin domain (aa 194 - 446). Mature human Olfactomedin-2 shares 98% aa identity with rat, bovine and equine, 97% with mouse and canine, 81% with Xenopus, and 77% with zebrafish Olfactomedin-2. No alternate splice variants have been reported for Olfactomedin-2 (1). Polymorphisms of human Olfactomedin-2, with or without additional polymorphisms in optineurin (OPTN), have been associated with a small fraction of high intraocular pressure or open-angle glaucoma cases in Japanese patients (6). The family member myocilin shows stronger association with these ocular disorders, and either myocilin or Noelin-1 might heterodimerize with Olfactomedin-2 (1). Evidence suggests that Olfactomedin-2 has evolved from Noelin-1 gene duplication, and myocilin from Olfactomedin-2 gene duplication (1).

• References:

1. Mukhopadhyay, A. et al. (2004) Mol. Vis. 10:304.

2. Kulkarni, N.H. et al. (2000) Genet. Res. Camb. 76:41.

3. Tomarev, S.I. and N. Nakaya (2009) Mol. Neurobiol. 40:122.

4. Moreno, T.A. & M. Bronner-Fraser (2002) Mech. Dev. 119:121.

5. Lee, J-A. et al. (2008) Mech. Dev. 125:167.

6. Funayama, T. et al. (2006) Invest. Ophthalmol. Vis. Sci. 47:5368.

• Entrez Gene IDs:

  93145 (Human); 244723 (Mouse); 313783 (Rat)

• Alternate Names:

  neuronal olfactomedin related ER localized protein 2; NOE2; NOE2noelin 2; NOELIN2; Noelin-2; NOELIN2_V1; olfactomedin 2; Olfactomedin2; Olfactomedin-2; OlfC; OLFM2; OM2