• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of Neuro?2A mouse neuroblastoma cells. The ED    ₅₀ for this effect is 0.7-2.8 μg/mL.

• Source

  Mouse myeloma cell line, NS0-derived Ser21-Thr834, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Ser21

• Predicted Molecular Mass

  92.2 kDa

• SDS-PAGE

  110-120 kDa, reducing condtitions

Background: Calsyntenin-2

Calsyntenin-2, also called Alc-gamma (Alcadein-gamma; gene name CLSTN2) is a 120-135 kDa glycoprotein that is a member of the Alcadein family of cadherin domain?containing type I transmembrane molecules (1, 2). Mature human Calsyntenin-2 is a 935 amino acid (aa) protein with two extracellular cadherin domains. Proteolytic cleavage by ADAM10 and gamma -secretase generates a 105-110 kDa extracellular domain termed soluble alc-gamma, and a 25 kDa, highly acidic, calcium-binding intracellular domain termed Alc gamma ICD (1-3). The soluble alc-gamma portion of human Calsyntenin-2 (aa 21-831) shares 95%, 94%, 96%, 95% and 94% aa identity with mouse, rat, canine, bovine and equine Calsyntenin-2, respectively. A potential isoform variant shows a premature truncation after Gln608. Calsyntenins are predominantly expressed in the ER/Golgi and post-synaptic plasma membranes of neurons (1). While nearly all neurons express Calsyntenin 1, Calsyntenin-2 is mainly found in subpopulations GABAergic  neurons of cortical layers 5 and 6, cerebellum (in Purkinje cells), amygdale (basolateral cells), hippocampus (pyramidal cells and hilus interneurons) and some brain stem neurons (1). Calsyntenin-2 interacts with amyloid precursor protein (APP) through its binding protein, APBA2/X11L, where it is thought to regulate APP cleavage, and likely impacts post-synaptic signaling and gene activation (2, 3). Notably, Alc gamma ICD binds APBA1/FE65 and antagonizes amyloid intracellular domain (AICD) gene activation (3). Polymorphisms of the Calsyntenin-2 gene, in combination with polymorphisms of the brain signaling regulator KIBRA, are thought to modulate episodic memory performance (4, 5).

• References:

1. Hintsch, G. et al. (2002) Mol. Cell Neurosci. 21:393.

2. Hata, S. et al. (2009) J. Biol. Chem. 284:36024.

3. Araki, Y. et al. (2004) J. Biol. Chem. 279:24343.

4. Jacobsen, L.K. et al. (2009) Biol. Psychiatry 65:671.

5. Preuschhof, C. et al. (2010) Neuropsychologia 48:402.

• Entrez Gene IDs:

  64084 (Human); 64085 (Mouse); 171394 (Rat)

• Alternate Names:

  alcadein gamma; Alcadein-gamma; alcagamma; ALC-GAMMA; cadherin-related family member 13; calsyntenin 2; Calsyntenin2; Calsyntenin-2; CDHR13; CLSTN2; CS2; CS2alcadein-gamma; CSTN2; FLJ39113; FLJ39499; MGC119560