• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <1.0 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of Neuro?2A mouse neuroblastoma cells. When 5 x 10    ⁴ cells/well are added to Recombinant Human SALM3/LRFN4 coated plates (2.5 μg/mL, 100 μL/well). approximately 50%-70% will adhere after 30 minutes at 37 °C.    
     Optimal dilutions should be determined by each laboratory for each application.

• Source

  Chinese Hamster Ovary cell line, CHO-derived Cys17-Leu518 with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Cys17

• Predicted Molecular Mass

  54.2 kDa

• SDS-PAGE

  70-90 kDa, reducing conditions

Background: SALM3/LRFN4

Synaptic adhesion-like molecule 3 (SALM3; also leucine-rich repeat and fibronectin type-III domain-containing protein 4 (Lrfn4) is an approximately 90 kDa member of the Lrfn family of type I transmembrane glycoproteins (1).  Human SALM3 is synthesized as a 635 amino acid (aa) precursor that contains a 16 aa signal sequence, a 502 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 96 aa cytoplasmic region.  The ECD consists of seven leucine-rich repeats (LRR), an IgC2?like domain, and a fibronectin type-III domain, tandemly aligned in that order (1-2).  In addition, there are six potential sites for N-linked glycosylation. The C?terminal region contains an intracellular PDZ binding domain, which is conserved among SALMs 1-3, but is absent in SALMs 4 and 5 (3). Mature human SALM3 shares 97% aa sequence identity with mature mouse SALM3.  Northern blot analysis showed that in mice, SALM3 is strongly expressed in the adult brain and is also present in the adult testis (1). It is distributed throughout the neuron, including the growth cone (3).  In the developing mouse embryo, a temporal expression profile blot revealed a general increment of expression around E10.5, with weak expression detected before E10.5 (1). SALM3, like the other SALMs, promotes neurite outgrowth (3).  Specifically, the SALMs modify total outgrowth and neurite branching (3).  SALM3 may also be involved in synapse formation, synaptic maintenance, and other cellular interactions (3).

• References:

1. Morimura, N. et al. (2006) Gene 380:72.

2. Wang, C.-Y. et al. (2006) J. Neurosci. 26:2174.

3. Wang, P.Y. et al. (2008) Mol. Cell. Neurosci. 39:83.

• Long Name:

  Synaptic Adhesion-like Molecule 3/Leucine-rich Repeat and Fibronectin Type III Domain Containing 4

• Entrez Gene IDs:

  78999 (Human); 225875 (Mouse); 688721 (Rat)

• Alternate Names:

  FIGLER6; LRFN4; SALM3