• Species Reactivity

  Human

• Specificity

  Detects human Aiolos/IKZF3 in direct ELISAs.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 914821

• Purification

  Protein A or G purified from cell culture supernatant

• Immunogen

  E. coli-derived recombinant human Aiolos/IKZF3    
  Met1-Gly54    
  Accession # Q9UKT9

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 μg/10    ⁶ cells

  Human peripheral blood mononuclear cells (PBMCs) fixed and permeabilized with with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Aiolos/IKZF3

IKAROS family zinc finger 3 (IKZF3), also known as Aiolos, is a hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation.

• Long Name:

  DNA-binding Protein Aiolos

• Entrez Gene IDs:

  22806 (Human); 22780 (Mouse); 303511 (Rat)

• Alternate Names:

  AIO; Aiolos; IKAROS family zinc finger 3 (Aiolos); Ikaros family zinc finger protein 3; IKZF3; zinc finger protein Aiolos; ZNFN1A3; ZNFN1A3subfamily 1A, 3 (Aiolos)