• Species Reactivity

  Human

• Specificity

  Detects human Lgr4/GPR48 in Lgr4-transfected cell-based ELISA. Stains human Lgr4/GPR48 transfected but not irrelevant transfected cells in flow cytometry.    
   

• Source

  Monoclonal Mouse IgG    _2B Clone # 852229

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  NS0 mouse myeloma cell line transfected with human Lgr4/GPR48    
  Met1-Asp951    
  Accession # Q9BXB1

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25 μg/10    ⁶ cells

  See below

• 
  

• Blockade of Receptor-ligand Interaction

  In a functional flow cytometry test, 2.5 μg/mL of Mouse Anti-Human Lgr4/GPR48 Monoclonal Antibody completely blocks binding of biotinylated Recombinant Human R-Spondin 4 (100 ng/mL) to HEK293 human embryonic kidney cell line transfected with human Lgr4/GPR48.

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Immunocytochemistry

  8-25 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lgr4/GPR48

Lgr4 (Leucine-rich repeat GPR 4), also known as GPR48 (G-Protein-coupled Receptor 48), is a seven-transmembrane glycoprotein receptor in the Lgr family of cell surface receptors (1, 2). While this family includes receptors for hormones such as LH, FSH, TSH, and HCG, the subfamily comprising Lgr4, Lgr5, and Lgr6 are G-protein-independent mediators of the potentiating effect of R-Spondins on Wnt signaling (1-6). Lgr4 binds and forms complexes with R-Spondins, Frizzled Wnt receptors and LRP Wnt co-receptors (5). It acts at least in part by enhancing Wnt-dependent LRP phosphorylation, internalization of LRPs, and accumulation of beta -catenin (3, 4). Human Lgr4 cDNA encodes 951 amino acids (aa), including a long N-terminal Extracellular Domain (ECD, aa 25-544) with 16-17 LRR domains that mediate ligand interaction (1). The LRR-containing ECD of human Lgr4 shares 93% aa sequence identity with mouse, rat and bovine Lgr4, and 50-60% aa identity with human Lgr5 and Lgr6. Lgr4 is widely expressed in both embryo and adult. Expression of Lgr4 mRNA in adult humans is highest in pancreas, followed by liver, heart, muscle, brain, and placenta (1). In rodents, embryonic and adult expression includes liver, kidney, adrenals, bone/cartilage, and heart (2, 7-9). Lgr4 deletion in the mouse affects development in areas of expression, for example, inhibiting fetal liver definitive erythropoiesis (9). Deletion of Lgr4 specifically from stem and progenitor cells in intestinal crypts induces loss of crypts due to insufficient Wnt signaling (5, 6). Lgr4 may be over-expressed in carcinomas and may promote invasiveness and metastasis by down-regulating p27^Kip1 expression (10).

• References:

1. Loh, E.D. et al. (2001) Biochem. Biophys. Res. Commun. 282:757.

2. Hsu, S.Y. et al. (1998) Mol. Endocrinol. 12:1830.

3. Carmon, K.S. et al. (2011) Proc. Natl. Acad. Sci. USA 108:11452.

4. Glinka, A. et al. (2011) EMBO Rep. 12:1055.

5. de Lau, W. et al. (2011) Nature 476:293.

6. Ruffner, H. et al. (2012) PLoS ONE 7:e40975.

7. Van Schoore, G. et al. (2005) Histochem. Cell Biol. 124:35.

8. Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:2241.

9. Song, H. et al. (2008) J. Biol. Chem. 283:36687.

10. Gao, Y. et al. (2006) Cancer Res. 66:11623.

• Long Name:

  Leucine-rich Repeat Containing G Protein-coupled Receptor 4

• Entrez Gene IDs:

  55366 (Human); 107515 (Mouse); 286994 (Rat)

• Alternate Names:

  GPR48; GPR48G protein-coupled receptor 48; G-protein coupled receptor 48; leucine-rich repeat containing G protein-coupled receptor 4; Lgr4