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Human ROR gamma t/RORC2/NR1F3 MAb (Clone 1181A) 100 UG

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Human ROR gamma t/RORC2/NR1F3 MAb (Clone 1181A) 100 UG信息二維碼

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產(chǎn)品介紹

    基本參數(shù)

    詳細說明

    • Species Reactivity

      Human

    • Specificity

      Detects recombinant human ROR gamma t/RORC2/NR1F3 by direct ELISAs.

    • Source

      Recombinant Monoclonal Rabbit IgG Clone # 1181A

    • Purification

      Protein A or G purified from cell culture supernatant

    • Immunogen

      E.coli derived recombinant human ROR gamma T/RORC2/NR1F3    
      Met1-Gln100    
      Accession # P51449

    • Formulation

      Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

    • Label

      Unconjugated

    Applications

    • Recommended    
      Concentration

      Sample

    • Intracellular Staining by Flow Cytometry

      0.25 μg/10    6 cells

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Intracellular Staining by Flow Cytometry      
         

    Detection of ROR gamma /RORC/NR1F3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) unstimulated or (B) stimulated to induce Th17 cells were stained with Rabbit Anti-Human ROR gamma /RORC/NR1F3 Monoclonal Antibody (Catalog # MAB61093) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # ) and Mouse Anti-Human CD4 APC?conjugated Monoclonal Antibody (Catalog # ). Quadrant markers were set based on control antibody staining (Catalog # ). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # ). View our protocol for .

    Preparation and Storage

    • Reconstitution

      Reconstitute at 0.5 mg/mL in sterile PBS.

    • Shipping

      The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

    • Stability & Storage

      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

      • 12 months from date of receipt, -20 to -70 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

      • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: ROR gamma t/RORC2/NR1F3

    Retinoic acid-related Orphan Receptor gamma (ROR gamma, TOR, RORC; NR1F3) is a member of the orphan nuclear receptor family. ROR gamma is expressed in the muscle, thymus, testis, pancreas, prostate, heart, and liver. ROR gamma plays a role in thymocyte development and homeostasis. RORs bind to DNA as monomers on half-site elements with 5' A/T-rich extensions. An N-terminal isoform of ROR gamma, ROR gamma t, has been shown to be specifically expressed in the thymus.

    • Long Name:

      Retinoic Acid-Related Orphan Receptor gamma t

    • Entrez Gene IDs:

      6097 (Human)

    • Alternate Names:

      ROR gamma t; RORC2










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