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Human HSP47 MAb (Clone 950811) EA

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Human HSP47 MAb (Clone 950811) EA信息二維碼

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產(chǎn)品介紹

    基本參數(shù)

    詳細說明

    • Species Reactivity

      Human

    • Specificity

      Detects human HSP47 in direct ELISAs and Western blots.  In Western blots, no cross-reactivity with mouse HSP47 is observed.    
       

    • Source

      Monoclonal Mouse IgG    2B Clone # 950811

    • Purification

      Protein A or G purified from hybridoma culture supernatant

    • Immunogen

      Chinese hamster ovary cell line CHO-derived recombinant human HSP47    
      Ala19-Asp412    
      Accession # P50454

    • Formulation

      Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

    • Label

      Unconjugated

    Applications

    • Recommended    
      Concentration

      Sample

    • Western Blot

      0.1 μg/mL

      See below


    • Immunohistochemistry

      5-25 μg/mL

      See below


    • Immunocytochemistry

      5-25 μg/mL

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Western Blot      
         

    Detection of Human HSP47 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, A549 human lung carcinoma cell line, and OVCAR?3 human ovarian carcinoma cell line. PVDF membrane was probed with 0.1 μg/mL of Mouse Anti-Human HSP47 Monoclonal Antibody (Catalog # MAB91661) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). A specific band was detected for HSP47 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using .

    Immunocytochemistry      
         

    HSP47 in BG01V Human Embryonic Stem Cells. HSP47 was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human HSP47 Monoclonal Antibody (Catalog # MAB91661) at 10 μg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights? 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for .

    Immunohistochemistry      
         

    HSP47 in Human Breast Cancer Tissue. HSP47 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human HSP47 Monoclonal Antibody (Catalog # MAB91661) at 25 μg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # ) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for .

    Preparation and Storage

    • Reconstitution

      Reconstitute at 0.5 mg/mL in sterile PBS.

    • Shipping

      The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

    • Stability & Storage

      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

      • 12 months from date of receipt, -20 to -70 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

      • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: HSP47

    Heat Shock Protein 47 (HSP47), also known as Serpin-H1/CBP1/CBP2, is localized to endoplasmic reticulum (ER), where it is a collagen-specific molecular chaperone.  In the ER, HSP47 interacts with and stabilizes correctly-folded procollagen. Nucleotide polymorphisms may be associated with preterm birth and Osteogenesis Imperfecta type X.  Serpin-H1 is up-regulated in several cancers including squamous cell carcinoma, breast and prostate carcinomas.  Expression in tumors drives malignant growth and invasion by enhancing deposition of extracellular matrix proteins.

    • References:

      1. Christiansen HE, et al, (2010) Am. J. Hum. Genet. 86:3892.

      2. Tasab M, et al, (2000) EMBO J. 19:22043.

      3. Kwon YJ, et al, (2009) Oncol Res. 18:1414.

      4. Zhu J, et al, (2015) Cancer Res. 75:15805.

      5. Nese N, et al, (2010) Anal Quant Cytol Histol. 32:90

    • Long Name:

      Heat Shock Protein 47

    • Entrez Gene IDs:

      871 (Human); 12406 (Mouse); 29345 (Rat)

    • Alternate Names:

      (collagen binding protein 1); Arsenic-transactivated protein 3; AsTP3; BERF-1; CBP1; CBP2; CBP2RA-A47; Cell proliferation-inducing gene 14 protein; Collagen-binding protein; colligen; Colligin; colligin-1; colligin-2; gp46; HSP47; HSP47serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47); member 1, (collagen binding protein 1); member 2; member 2, (collagen-binding protein 2); OI10; PPROM; proliferation-inducing gene 14; RA-A47; rheumatoid arthritis antigen A-47,47 kDa heat shock protein; Rheumatoid arthritis-related antigen RA-A47; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47); Serpin H1; serpin peptidase inhibitor, clade H (heat shock protein 47), member 1; SERPINH1; SERPINH2colligin




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