• Species Reactivity

  Human

• Specificity

  Detects human TRIM38 in direct ELISAs.    
   

• Source

  Monoclonal Mouse IgG    _2B Clone # 933011

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E.coli-derived recombinant human TRIM38    
  Leu190-Ile414    
  Accession # O00635

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunohistochemistry

  5-25 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRIM38

Human Tripartite Motif 38 (TRIM38, RNF15) is a 465 aminoacids E3 ubiquitin ligase that plays a key role in tuning TLR-mediated immune responses by selectively targeting molecules in the TLR signaling for proteasomal degradation. The TRIM family of E3 ligases has more than 70 members in humans. Many members of this protein family are important immune regulators, mediating innate immune cell signal transduction and subsequent cytokine induction immune regulation. Trim38 deficiency, for example, greatly increases TLR3- and TLR4-mediated induction of type I Interferons and proinflammatory cytokines, such as TNF-alpha, IL-1 beta, and IL-6, in immune cells and in vivo.

• Long Name:

  Tripartite Motif-containing 38

• Entrez Gene IDs:

  10475 (Human); 214158 (Mouse); 680019 (Rat)

• Alternate Names:

  MGC8946; RING finger protein 15RORETRo/SSA ribonucleoprotein homolog; RNF15; RoRet; TRIM38; tripartite motif containing 38; tripartite motif-containing 38; tripartite motif-containing protein 38; Zinc finger protein RoRet; zinc-finger protein RoRet