• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie? Blue stain.

• Activity

  Recombinant Human His6-USP19 is a ubiquitin--specific deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application. We recommend an initial USP19 concentration of 10-100 nM.

• Source

  Spodoptera frugiperda,     Sf 21 (baculovirus)-derived Met1 - Arg1290, Contains a C-terminal 6-His tag

• Accession #

• Predicted Molecular Mass

  143 kDa

Background: USP19

Ubiquitin specific processing protease 19 (USP19) is a 146 kDa member of the C19 peptidase family that contains two alpha crystallin domains and one MYND-type zinc finger. USP19 plays a role in the rescue of ER-associated protein degradation (ERAD) substrates such as cystic fibrosis transmembrane conductance regulator (CFTR) delta F508 and T-cell receptor-alpha (TCR alpha ) from proteasomal degradation. USP19 also interacts with the ubiquitin ligases cellular IAP 1 (cIAP1) and cIAP2. Knockdown of USP19 decreases levels of both cIAPs, whereas overexpression of USP19 results in a marked increase in cIAP levels. Although it effectively removes ubiquitin from cIAPs in vitro, USP19 may stabilize cIAP proteins in vivo mainly through deubiquitinase-independent mechanisms. Intriguingly, partial rescue of TCR alpha (but not CFTR delta F508) in the ERAD pathway is also observed using a catalytically dead USP19, suggesting that USP19 exerts a non-catalytic function for select substrates in multiple biological pathways. This recombinant protein contains amino acids a C-terminal 6-His tag.

• References:

1. Hassink G.C., et al. (2009) EMBO Rep. 10: 755

2. Iph?fer, A. et al. (2012) ChemBioChem 13: 1416

3. Mei, Y. et al. (2011) J. Biol. Chem. 286: 35380

• Long Name:

  Ubiquitin Specific Protease 19

• Entrez Gene IDs:

  10869 (Human); 71472 (Mouse); 361190 (Rat)

• Alternate Names:

  Deubiquitinating enzyme 19; EC 3.1.2.15; EC 3.4.19.12; KIAA0891; Ubiquitin Carboxyl-Terminal Hydrolase 19; ubiquitin specific peptidase 19; ubiquitin thioesterase 19; Ubiquitin Thiolesterase 19; Ubiquitin-specific-processing protease 19; USP19; Zinc finger MYND domain-containing protein 9; ZMYND9; ZMYND9ubiquitin specific protease 19