• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie? Blue stain.

• Activity

  Reaction conditions will need to be optimized for each specific application. We recommend an initial His6-USP10 concentration of 20-100 nM in reactions utilizing Ubiquitin-AMC or Ubiquitin-Rhodamine substrates (U-550 or U-555).

• Source

  Spodoptera frugiperda,     Sf 21 (baculovirus)-derived Contains an C-terminal 6-His tag

• Accession #

• Predicted Molecular Mass

  88 kDa

Background: USP10

Ubiquitin carboxyl-terminal hydrolase 10 (USP10) is a specialized cysteine protease with a predicted molecular weight of 87 kDa. USP10 is a member of the peptidase C19 family and the human protein shares 83% amino acid sequence identity with its mouse ortholog. Reported USP10 targets include p53, BECN1, SNX3 and CFTR. USP10 translocates to the nucleus in response to DNA damage and deubiquitinates p53, thus regulating the p53-dependent DNA damage response. USP10 deubiquitination of BECN1 leads to stabilization of PIK3C3/VPS34-containing complexes which drives autophagy. The small molecule Spautin-1 exerts its potent inhibitory effects on autophagy partially through the inhibition of USP10 activity.

• References:

1. Boulkroun S., et al. (2008) Am. J. Physiol. 295:F889-F900

2. Liu J., et al. (2011) Cell 147: 223

3. Yuan J., et al. (2010) Cell 140: 384

• Long Name:

  Ubiquitin Specific Protease 10

• Entrez Gene IDs:

  9100 (Human); 22224 (Mouse); 307905 (Rat)

• Alternate Names:

  Deubiquitinating enzyme 10; EC 3.4.19.12; KIAA0190MGC2621; ubiquitin carboxyl-terminal hydrolase 10; ubiquitin specific peptidase 10; ubiquitin specific protease 10; ubiquitin thioesterase 10; Ubiquitin Thiolesterase 10; Ubiquitin-specific-processing protease 10; UBPO; Uchrp; USP10