• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie? Blue stain

• Activity

  Recombinant Human USP28 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP28 concentration of 10-500 nM.  

• Source

  Spodoptera frugiperda,     Sf 21 (baculovirus)-derived Contains a C-terminal 6-His tag

• Accession #

• Predicted Molecular Mass

  123 kDa

Background: USP28

USP28 is a deubiquitinating enzyme of the C19 peptidase family that regulates MYC stability and DNA damage response checkpoints. USP28 regulates DNA repair by deubiquitinating and thereby stabilizing CLSPN - a protein that is ubiquitinated by the APC/C E3 Ubiquitin ligase in a cell-cycle dependent manner. USP28 interacts with isoform 1 of FBXW7 in the nucleoplasm, and regulates MYC degradation by counteracting its ubiquitination. Recently, a potential role in HIF-1 alpha -dependent processes such as angiogenesis and metastasis has been described for this deubiquitinase. USP28 has been demonstrated to cleave K6, K11, K48, and K63-linked poly-Ubiquitin chains. This recombinant protein contains a C-terminal 6-His tag.

• References:

1. Basserman F.,         et al. (2008) Cell         134: 256-267.

2. Flügel D.,         et al. (2012) Blood         119:1292-301.

3. Popov N.,         et al. (2007) Cell Cycle         6: 2327-2331.

4. Zhang D.,         et al. (2006) Cell         126: 529-542.

• Long Name:

  Ubiquitin Specific Protease 28

• Entrez Gene IDs:

  57646 (Human); 235323 (Mouse); 315639 (Rat)

• Alternate Names:

  Deubiquitinating enzyme 28; EC 3.1.2.15; EC 3.4.19.12; KIAA1515ubiquitin carboxyl-terminal hydrolase 28; ubiquitin specific peptidase 28; ubiquitin specific protease 28; ubiquitin thioesterase 28; Ubiquitin thiolesterase 28; Ubiquitin-specific-processing protease 28; USP28