• Species Reactivity

  Rat

• Specificity

  Detects rat ALK-7 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Activin R1B is observed.

• Source

  Monoclonal Mouse IgG    _2A Clone # 118311

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant rat ALK?7    
  Leu26-Arg108    
  Accession # NP_620790

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Biotin

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  Recombinant Rat ALK?7 Fc Chimera (Catalog # )

• 
  

• Flow Cytometry

  2.5 μg/10    ⁶ cells

  Rat cortical stem cells

• 
  

• Immunohistochemistry

  8-25 μg/mL

  Perfusion fixed frozen sections of rat brain (hypothalamus)

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ALK-7

ALKs 1-7 are type I serine/threonine kinase receptors for TGF-beta superfamily members. They undergo hetero-oligomerization with type II serine/threonine kinase receptors to transduce signals, usually through Smad proteins. Alk-1 binds TGF-beta and Alk-7 is thought to bind nodal.

• Long Name:

  Activin Receptor-like Kinase 7

• Entrez Gene IDs:

  130399 (Human); 245921 (Rat)

• Alternate Names:

  activin A receptor, type IC; Activin receptor type IC; activin receptor type-1C; Activin receptor-like kinase 7; Activin RIC; ACTR-IC; ACVR1C; ACVRLK7; ALK7; ALK-7; ALK7ALK-7; EC 2.7.11; EC 2.7.11.30