• Species Reactivity

  Mouse

• Specificity

  Detects mouse OSM R beta.

• Source

  Monoclonal Rat IgG    _2A Clone # 118125

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse OSM R beta    
  Glu24-Leu738    
  Accession # O70458

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

• Label

  Biotin

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  2.5 μg/10    ⁶ cells

  See below

• 
  

• Immunohistochemistry

  8-25 μg/mL

  Immersion fixed frozen sections of mouse embryo (E13.5-15.5)

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OSM R beta

Oncostatin M (OSM) is a member of the IL-6 family of cytokines that share the gp130 as a common signal transducing receptor subunit. Human OSM signals through two types of human OSM receptor complexes: the type I complex comprising the Leukemia Inhibitory Factor Receptor beta (LIF R beta ) and gp130, the type II complex made up of OSM Receptor beta (OSM R beta ) and gp130. In contrast, mouse OSM signals only through the mouse OSM R beta and gp130 complex. Mouse OSM R beta cDNA encodes a 971 amino acid (aa) residue type I transmembrane protein which contains a 23 aa residue signal peptide, an extracellular domain of 714 aa, a transmembrane domain of 20 aa and a 214 aa cytoplasmic domain. Mouse OSM R beta alone binds mOSM with low-affinity, but forms a high-affinity binding complex in the presence of gp130. Mouse OSM R beta is 55% identical at the amino acid sequence level to human OSM R beta.

• References:

1. Lindberg, R.A. et al. (1998) Mol. Cell. Biol. 18:3357.

2. Tanaka, M. et al. (1999) Blood 93:804.

• Long Name:

  Oncostatin M Receptor beta

• Entrez Gene IDs:

  9180 (Human); 18414 (Mouse); 310132 (Rat)

• Alternate Names:

  OSM R beta; OSMR; OSMRB