• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Activity

  Measured by its binding ability in a functional ELISA. Immobilized rmEphA7/Fc Chimera at 2 μg/mL (100 μL/well) can bind biotinylated rmEphrin-A4/Fc Chimera with a linear range of 0.16-10 ng/mL.    
     Optimal dilutions should be determined by each laboratory for each application.  

• Source

  Mouse myeloma cell line, NS0-derived

  Human Ephrin-A4 (Leu26 - Gly176) Accession # Q3UQB5	DIEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Leu26

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  43.6 kDa (monomer)

• SDS-PAGE

  50-60 kDa, reducing conditions

Background: Ephrin-A4

Ephrin-A4, also known as LERK-4 and EFL-4 (1), is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A4 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1 (2, 3). The extracellular domains of human and mouse ephrin-A4 share 80% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

• References:

1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.

2. Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.

3. Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.

• Entrez Gene IDs:

  1945 (Human); 13639 (Mouse)

• Alternate Names:

  EFL4; EFL-4; EFNA4; EPH-related receptor tyrosine kinase ligand 4; EphrinA4; Ephrin-A4; EPLG4MGC125826; LERK-4; LERK4FLJ57652; ligand of eph-related kinase 4