• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <1.0 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse EphB2 Fc Chimera at 2 μg/mL (100 μL/well) can bind Biotinylated Recombinant Mouse Ephrin-B2 Fc Chimera with a linear range of 0.039-2.5 ng/mL.    
     Optimal dilutions should be determined by each laboratory for each application.  

• Source

  Mouse myeloma cell line, NS0-derived

  Mouse Ephrin-B2 (Arg27 - Ala227) Accession # AAA82934	DIEGRMD	Human IgG1 (Pro100 - Lys330)	6-His tag
  N-terminus			C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Arg27

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  49.6 kDa (monomer)

• SDS-PAGE

  60-65 kDa, reducing conditions

Background: Ephrin-B2

Ephrin?B2, also known as Htk?L, ELF?2, LERK?5, and NLERK?1, is a 40 kDa member of the Ephrin?B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains of Ephrin?B ligands are structurally related to GPI?anchored Ephrin?A ligands. Eph?Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin?B2 preferentially interacts with receptors in the EphB family (1, 2). Mature mouse Ephrin?B2 consists of a 204 amino acid (aa) extracelluar domain (ECD), a 21 aa transmembrane segment, and an 83 aa cytoplasmic domain [Cerretti 1197, Bergemann 4921, Bennett 1866]. Within the ECD, mouse Ephrin?B2 shares 97% and 98% aa sequence identity with human and rat Ephrin?B2, respectively. Ephrin?B2 is expressed presynaptically on neurons (6, 7). It promotes presynaptic development, EphB2 shedding, axonal growth cone collapse, and neurite repulsion, and also regulates inflammatory and neuropathic pain (6 ? 8). Ephrin?B2 is expressed by vascular mural cells and arterial vascular and lymphatic endothelium (9, 10). It exerts proliferative and migratory effects on these cells during angiogenesis and lymphangiogenesis in part by regulating the signaling activity of VEGF R2 and VEGF R3 (8 ? 11). Ephrin?B2 plays a role in the immune response by mediating monocyte extravasation and T cell costimulation (12, 13). It is upregulated in invasive cancers and promotes tumor cell migration, invasion, and tumor angiogenesis (14  ? 16). It functions as a cellular entry receptor for Hendra and Nipah viruses (17). Ephrin?B2 is also important for the separation of the urinary and intestinal tracts during development (18).

• References:

1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.

2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.

3. Cerretti, D.P. et al. (1995) Mol. Immunol. 32:1197.

4. Bergemann, A.D. et al. (1995) Mol. Cell. Biol. 15:4921.

5. Bennett, B.D. et al. (1995) Proc. Natl. Acad. Sci. 92:1866.

6. McClelland, A.C. et al. (2009) Proc. Natl. Acad. Sci. 106:20487.

7. Zhao, J. et al. (2010) Mol. Pain 6:77.

8. Lin, K.-T. et al. (2008) J. Biol. Chem. 283:28969.

9. Foo, S.S. et al. (2006) Cell 124:161.

10. Wang, Y. et al. (2010) Nature 465:483.

11. Sawamiphak, S. et al. (2010) Nature 465:487.

12. Pfaff, D. et al. (2008) J. Cell Sci. 121:3842.

13. Yu, G. et al. (2003) J. Immunol. 171:106.

14. Meyer, S. et al. (2005) Int. J. Oncol. 27:1197.

15. Nakada, M. et al. (2010) Int. J. Cancer 126:1155.

16. Liu, W. et al. (2004) Br. J. Cancer 90:1620.

17. Bonaparte, M.I. et al. (2005) Proc. Natl. Acad. Sci. 102:10652.

18. Dravis, C. et al. (2004) Dev. Biol. 271:272.

• Entrez Gene IDs:

  1948 (Human); 13642 (Mouse); 30219 (Zebrafish)

• Alternate Names:

  EFNB2; ELF-2; EphrinB2; Ephrin-B2; Htk-L; LERK-5; NLERK-1